Detailed information of TA system TA099928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 987265..987803
• Replicon: chromosome
• Accession: NZ_CP027712
• Organism: Pseudomonas chlororaphis subsp. chlororaphis strain DSM 50083

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A285H8D2
• Locus tag: C4K27_RS04310
• Protein ID: WP_009042149.1
• Coordinates: 987525..987803 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4K27_RS04305
• Protein ID: WP_053259626.1
• Coordinates: 987265..987528 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K27_RS04290	983254..985527	-	2274	WP_053259624.1	TonB-dependent siderophore receptor	-
C4K27_RS04295	985734..986414	+	681	WP_009042146.1	Fe2+-dependent dioxygenase	-
C4K27_RS04300	986418..987203	+	786	WP_053259625.1	sel1 repeat family protein	-
C4K27_RS04305	987265..987528	+	264	WP_053259626.1	ribbon-helix-helix domain-containing protein	Antitoxin
C4K27_RS04310	987525..987803	+	279	WP_009042149.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K27_RS04315	987878..989041	-	1164	WP_009042150.1	type III PLP-dependent enzyme	-
C4K27_RS04320	989741..990898	-	1158	WP_007923834.1	ABC transporter ATP-binding protein	-
C4K27_RS04325	990895..991548	-	654	WP_053259627.1	ABC transporter permease	-
C4K27_RS04330	991563..992456	-	894	WP_053259628.1	glycine betaine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Ribbon-helix-helix protein, CopG (7-44)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10853.45 Da
• Isoelectric Point: 8.5409

>T99928 WP_009042149.1 NZ_CP027712:987525-987803 [Pseudomonas chlororaphis subsp. chlororaphis]
VRELKWTSKALSDLARLFEFLAPVNRSVAARTVQSLTQAPDTLLTNPRIGEQLEEFQPRDVRRLLVGPYEMRYEIQDATL
YILRVWHVREDR

Nucleotide

• Length: 279 bp

>T99928 NZ_CP027712:987525-987803 [Pseudomonas chlororaphis subsp. chlororaphis]
GTGAGGGAATTGAAATGGACGAGCAAGGCACTGTCGGACCTGGCCCGACTGTTTGAGTTTCTTGCTCCGGTGAACCGTTC
GGTGGCAGCTCGAACTGTGCAATCGCTCACGCAGGCCCCGGATACCTTGCTGACCAATCCGCGTATCGGCGAACAGTTGG
AGGAGTTTCAGCCTCGCGATGTTCGGCGTTTATTGGTCGGGCCTTACGAGATGCGTTACGAGATCCAGGATGCGACGCTC
TACATCCTGCGAGTGTGGCATGTACGAGAAGACCGCTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9769.19 Da
• Isoelectric Point: 5.6088

>AT99928 WP_053259626.1 NZ_CP027712:987265-987528 [Pseudomonas chlororaphis subsp. chlororaphis]
MTATKVLTAHVPLELADKVDQMASRLERSRGWIVKQALVAWVDQEEERSRLTHEALADVDVGRVIDHQAVQAWAESLMSD
KPLPVPK

Nucleotide

• Length: 264 bp

>AT99928 NZ_CP027712:987265-987528 [Pseudomonas chlororaphis subsp. chlororaphis]
ATGACCGCAACTAAAGTGCTGACCGCACATGTGCCGTTGGAGTTGGCCGACAAGGTCGACCAGATGGCCTCTCGGCTGGA
GCGCTCGCGCGGCTGGATCGTCAAGCAGGCTCTGGTGGCGTGGGTCGATCAGGAAGAGGAACGCAGTCGCCTGACCCATG
AGGCTTTGGCCGATGTGGATGTTGGGCGGGTCATCGATCACCAGGCGGTCCAGGCATGGGCTGAAAGCCTGATGAGTGAC
AAGCCTTTGCCGGTGCCCAAGTGA

Structures

Toxin

• AlphaFold DB: A0A285H8D2