Detailed information of TA system TA099927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 318701..319314
• Replicon: chromosome
• Accession: NZ_CP027712
• Organism: Pseudomonas chlororaphis subsp. chlororaphis strain DSM 50083

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K27_RS01380
• Protein ID: WP_053259259.1
• Coordinates: 318701..318883 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2N8BII0
• Locus tag: C4K27_RS01385
• Protein ID: WP_009041686.1
• Coordinates: 318913..319314 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K27_RS01355	314841..315098	-	258	WP_053259255.1	hypothetical protein	-
C4K27_RS01360	315338..316234	-	897	WP_007925288.1	SDR family oxidoreductase	-
C4K27_RS01365	316264..316773	-	510	WP_053259256.1	DUF1097 domain-containing protein	-
C4K27_RS01370	316807..317547	-	741	WP_053259257.1	SDR family oxidoreductase	-
C4K27_RS01375	317686..318576	+	891	WP_053259258.1	LysR family transcriptional regulator	-
C4K27_RS01380	318701..318883	+	183	WP_053259259.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K27_RS01385	318913..319314	+	402	WP_009041686.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K27_RS01390	319403..320449	-	1047	WP_053259260.1	LacI family DNA-binding transcriptional regulator	-
C4K27_RS01395	320656..322914	+	2259	WP_053259261.1	TonB-dependent receptor	-
C4K27_RS01400	322961..324286	+	1326	WP_009041689.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6959.11 Da
• Isoelectric Point: 10.2817

>T99927 WP_053259259.1 NZ_CP027712:318701-318883 [Pseudomonas chlororaphis subsp. chlororaphis]
MRSRELIRMIEEDGWYLIAVKGSHHQYKYLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Nucleotide

• Length: 183 bp

>T99927 NZ_CP027712:318701-318883 [Pseudomonas chlororaphis subsp. chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAGTA
CAAGTATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14629.48 Da
• Isoelectric Point: 4.7569

>AT99927 WP_009041686.1 NZ_CP027712:318913-319314 [Pseudomonas chlororaphis subsp. chlororaphis]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAEAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGMI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT99927 NZ_CP027712:318913-319314 [Pseudomonas chlororaphis subsp. chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGCCATACCGCGGCAGAAGCCTTCGAGAATGTGAAAGAGGCCTTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAACCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAACCCCGATTACGCCGGTGGCGTGTGGGGCATGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGCTTCAACGCCACCCTGCCGGAGCAATTGCTGGAACGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGCTCGCGTTCGGGCTTTCTCGCCGCGGCGGCATTGCGCGAGTTATCGGCGT
AG

Structures

Antitoxin

• AlphaFold DB: A0A2N8BII0