Detailed information of TA system TA099926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 213093..213609
• Replicon: chromosome
• Accession: NZ_CP027712
• Organism: Pseudomonas chlororaphis subsp. chlororaphis strain DSM 50083

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A285EY61
• Locus tag: C4K27_RS00900
• Protein ID: WP_009041601.1
• Coordinates: 213328..213609 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4K27_RS00895
• Protein ID: WP_081002203.1
• Coordinates: 213093..213338 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K27_RS00870	208847..209851	+	1005	WP_053259189.1	aspartyl beta-hydroxylase	-
C4K27_RS00875	209817..210575	-	759	WP_007932057.1	slipin family protein	-
C4K27_RS00880	210577..211962	-	1386	WP_053259190.1	nodulation protein NfeD	-
C4K27_RS00885	212186..212647	+	462	WP_053259191.1	YbaK/EbsC family protein	-
C4K27_RS00890	212785..213030	+	246	WP_009041599.1	DUF2789 domain-containing protein	-
C4K27_RS00895	213093..213338	+	246	WP_081002203.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
C4K27_RS00900	213328..213609	+	282	WP_009041601.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K27_RS00905	213741..214169	+	429	WP_053259193.1	winged helix DNA-binding protein	-
C4K27_RS00910	214166..216250	+	2085	WP_053259194.1	FUSC family protein	-
C4K27_RS00915	216247..216456	+	210	WP_007932080.1	DUF1656 domain-containing protein	-
C4K27_RS00920	216453..217349	+	897	WP_053259195.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10879.81 Da
• Isoelectric Point: 10.5797

>T99926 WP_009041601.1 NZ_CP027712:213328-213609 [Pseudomonas chlororaphis subsp. chlororaphis]
MTYKLQFLPSARKEWDKLGHTLREQFKKKLAERLEMPRVPADALHGMADCYKIKLKASGYRLVYQVIEDQVVVSVVAVGK
RERSAVYERARKR

Nucleotide

• Length: 282 bp

>T99926 NZ_CP027712:213328-213609 [Pseudomonas chlororaphis subsp. chlororaphis]
ATGACTTATAAGCTCCAGTTTCTACCTTCGGCGCGCAAGGAATGGGACAAGCTCGGTCATACCCTACGCGAGCAATTCAA
GAAGAAGCTGGCCGAGCGCCTGGAAATGCCACGGGTGCCCGCGGATGCCCTTCACGGCATGGCGGACTGCTACAAGATCA
AGCTCAAAGCCTCTGGTTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTCGTGGTTTCGGTGGTGGCCGTGGGTAAA
CGTGAACGCAGTGCCGTGTATGAGCGGGCACGAAAGCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8843.18 Da
• Isoelectric Point: 4.7164

>AT99926 WP_081002203.1 NZ_CP027712:213093-213338 [Pseudomonas chlororaphis subsp. chlororaphis]
MQSVLADKAVSVSELKKNPSAVLSSAQGGPVAVLNHNRVMGYMVPADVFEAMMERLDDLELAEIVRARRHETPVPVNLDD
L

Nucleotide

• Length: 246 bp

>AT99926 NZ_CP027712:213093-213338 [Pseudomonas chlororaphis subsp. chlororaphis]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAATCCTTCGGCTGTCCTGAGCAGCGCACA
GGGTGGTCCGGTTGCGGTGCTCAATCACAATCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCGATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGTCACGAGACCCCGGTCCCGGTAAATCTGGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: A0A285EY61