Detailed information of TA system TA099899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 6263875..6264464
• Replicon: chromosome
• Accession: NZ_CP027707
• Organism: Pseudomonas chlororaphis subsp. piscium strain DSM 21509

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C4K38_RS28440
• Protein ID: WP_016702674.1
• Coordinates: 6264165..6264464 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: C4K38_RS28435
• Protein ID: WP_053281098.1
• Coordinates: 6263875..6264168 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K38_RS28410	6259510..6260238	+	729	WP_053281093.1	ABC transporter permease	-
C4K38_RS28415	6260235..6260945	+	711	WP_053281094.1	ABC transporter permease	-
C4K38_RS28420	6260962..6261726	+	765	WP_053281095.1	ATP-binding cassette domain-containing protein	-
C4K38_RS28425	6262113..6262505	+	393	WP_053281096.1	DUF3077 domain-containing protein	-
C4K38_RS28430	6262706..6263869	+	1164	WP_053281097.1	GGDEF domain-containing protein	-
C4K38_RS28435	6263875..6264168	-	294	WP_053281098.1	putative addiction module antidote protein	Antitoxin
C4K38_RS28440	6264165..6264464	-	300	WP_016702674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K38_RS28445	6264637..6265086	+	450	WP_053281099.1	DUF3828 domain-containing protein	-
C4K38_RS28450	6265111..6266121	-	1011	WP_172833187.1	NAD(P)-dependent alcohol dehydrogenase	-
C4K38_RS28455	6266177..6266476	-	300	WP_053281101.1	ArsR family transcriptional regulator	-
C4K38_RS28460	6266543..6267631	-	1089	WP_053281102.1	nitrite reductase, copper-containing	-
C4K38_RS28465	6267859..6268101	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
C4K38_RS28470	6268098..6268985	+	888	WP_053281103.1	(2E,6E)-farnesyl diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11221.93 Da
• Isoelectric Point: 10.6086

>T99899 WP_016702674.1 NZ_CP027707:c6264464-6264165 [Pseudomonas chlororaphis subsp. piscium]
MIEIKQTATFMEWENRLKDSRARALIAARIFRLANGLAGDVSPVGEGVSELRIHYGPGYRVYFQQLGSQLVILLCGGDKS
RQSRDIELAKQLARRWSRS

Nucleotide

• Length: 300 bp

>T99899 NZ_CP027707:c6264464-6264165 [Pseudomonas chlororaphis subsp. piscium]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATCGC
TGCGCGGATTTTTCGCCTGGCCAACGGCCTTGCCGGTGACGTGTCACCGGTGGGCGAGGGCGTCAGTGAGCTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCTCGGTTCGCAACTTGTCATTCTGTTATGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAACAGCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10584.16 Da
• Isoelectric Point: 6.4627

>AT99899 WP_053281098.1 NZ_CP027707:c6264168-6263875 [Pseudomonas chlororaphis subsp. piscium]
MNEPLFDYDPATSLRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLDMTARVHVRP

Nucleotide

• Length: 294 bp

>AT99899 NZ_CP027707:c6264168-6263875 [Pseudomonas chlororaphis subsp. piscium]
ATGAACGAACCGTTATTCGACTATGACCCCGCCACGTCCCTACGCGACCCGCAGGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAGGCCCTGGGTGTGGTGGCGCGGGCCAAGGGTATGACCGAACTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGTTGTATCGCTCGTTCAGCGACAAGGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGTGCCCTGGGGCTGGACATGACGGCGCGGGTGCATGTCAGGCCGTAG