Detailed information of TA system TA099897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2349462..2350081
• Replicon: chromosome
• Accession: NZ_CP027707
• Organism: Pseudomonas chlororaphis subsp. piscium strain DSM 21509

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4K38_RS10960
• Protein ID: WP_053278351.1
• Coordinates: 2349899..2350081 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K38_RS10955
• Protein ID: WP_053278311.1
• Coordinates: 2349462..2349863 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K38_RS10940	2345647..2347536	+	1890	WP_053278308.1	PAS domain S-box protein	-
C4K38_RS10945	2347533..2348168	+	636	WP_053278309.1	response regulator transcription factor	-
C4K38_RS10950	2348261..2349010	+	750	WP_053278310.1	hypothetical protein	-
C4K38_RS10955	2349462..2349863	-	402	WP_053278311.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K38_RS10960	2349899..2350081	-	183	WP_053278351.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K38_RS10965	2350459..2350740	-	282	WP_053278312.1	hypothetical protein	-
C4K38_RS10970	2351158..2351514	-	357	WP_053278313.1	hypothetical protein	-
C4K38_RS10975	2351499..2351891	-	393	Protein_2123	hypothetical protein	-
C4K38_RS10980	2352023..2352304	-	282	WP_053278315.1	hypothetical protein	-
C4K38_RS10985	2353019..2354311	+	1293	WP_164487001.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6799.98 Da
• Isoelectric Point: 10.9678

>T99897 WP_053278351.1 NZ_CP027707:c2350081-2349899 [Pseudomonas chlororaphis subsp. piscium]
VQSRQLIKELEAAGWVLERVTGSHHLFKHPYRPETVPVPHPKKDLPRGTVRAIQKLAGLL

Nucleotide

• Length: 183 bp

>T99897 NZ_CP027707:c2350081-2349899 [Pseudomonas chlororaphis subsp. piscium]
GTGCAAAGCAGACAGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTT
CAAGCATCCCTACCGGCCCGAGACCGTTCCGGTGCCTCACCCGAAGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGTTATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14591.59 Da
• Isoelectric Point: 4.6017

>AT99897 WP_053278311.1 NZ_CP027707:c2349863-2349462 [Pseudomonas chlororaphis subsp. piscium]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELAREGQAIPLPSPTGIHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT99897 NZ_CP027707:c2349863-2349462 [Pseudomonas chlororaphis subsp. piscium]
GTGCAATATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCCACCGGCATCCAGATTCCTGACATTCCGGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAACTGGCCCGTG
AGGGGCAAGCCATTCCCCTGCCCTCGCCCACCGGCATACACCGCGCCAACCCCGACTTCGAGGGGATGGGCTGGGGCATG
CTGGATATCGATATCACGCCTTACCTGGGCAAAACCGAAAAGGTCAATGTGACCCTGCCGGGTTACGTGATTCAACGAAT
CGACCGTTTTGTGCGGGAGCACAACATCAAGAGCCGGTCGTCTTTCCTGGCGGATGCGGCGATGGAAAAGCTGGGGCGTT
AA