Detailed information of TA system TA099894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4916141..4916772
• Replicon: chromosome
• Accession: NZ_CP027706
• Organism: Pseudomonas sp. CMR12a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C4K39_RS22900
• Protein ID: WP_124347476.1
• Coordinates: 4916374..4916772 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G7A756
• Locus tag: C4K39_RS22895
• Protein ID: WP_068588739.1
• Coordinates: 4916141..4916374 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K39_RS22865	4911423..4911818	-	396	WP_124347473.1	GNAT family N-acetyltransferase	-
C4K39_RS22870	4911979..4912809	+	831	WP_124347474.1	amidohydrolase family protein	-
C4K39_RS22875	4912957..4913400	+	444	WP_068588728.1	DUF3592 domain-containing protein	-
C4K39_RS22880	4913405..4913989	+	585	WP_068588731.1	hypothetical protein	-
C4K39_RS22885	4914015..4915262	-	1248	WP_124347475.1	aspartate aminotransferase family protein	-
C4K39_RS22890	4915383..4915964	-	582	WP_124348383.1	DedA family protein	-
C4K39_RS22895	4916141..4916374	+	234	WP_068588739.1	antitoxin	Antitoxin
C4K39_RS22900	4916374..4916772	+	399	WP_124347476.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C4K39_RS22910	4917055..4917558	-	504	WP_068588884.1	DUF2938 domain-containing protein	-
C4K39_RS22915	4917667..4918332	+	666	WP_068588881.1	ArsR family transcriptional regulator	-
C4K39_RS22920	4918548..4920593	+	2046	WP_124347478.1	c-type cytochrome	-
C4K39_RS22925	4920754..4921356	+	603	WP_124347479.1	hypothetical protein	-
C4K39_RS22930	4921362..4921727	-	366	WP_031320450.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14924.13 Da
• Isoelectric Point: 7.8510

>T99894 WP_124347476.1 NZ_CP027706:4916374-4916772 [Pseudomonas sp. CMR12a]
MFKYMLDTNICIFTIKNKPQQVREAFSRNQGRLCISSITLMELIYGAEKSAVPEKNLGRVESFVARLQVLDYDDNAAIHS
GQLQAELAQKGNRIGPYDQLIAGHARSLGLTLVTNNLREFERVPGLRVEDWV

Nucleotide

• Length: 399 bp

>T99894 NZ_CP027706:4916374-4916772 [Pseudomonas sp. CMR12a]
ATGTTCAAGTACATGCTCGATACCAATATCTGCATCTTTACCATCAAGAACAAACCCCAGCAGGTTCGCGAAGCGTTCAG
CCGTAATCAGGGGCGTTTGTGCATCAGTTCCATCACGTTGATGGAACTGATCTATGGTGCGGAAAAATCTGCCGTGCCGG
AGAAAAACCTGGGGCGTGTCGAAAGCTTCGTGGCACGCCTGCAAGTGCTGGATTATGACGATAACGCCGCGATCCATAGT
GGCCAGCTACAGGCCGAACTCGCCCAGAAGGGCAACCGAATAGGTCCCTACGATCAACTGATAGCGGGACATGCCAGGTC
ATTGGGGCTGACCCTGGTCACCAACAATCTTCGTGAGTTCGAGCGTGTGCCCGGGTTGCGCGTCGAGGATTGGGTCTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8732.69 Da
• Isoelectric Point: 4.2092

>AT99894 WP_068588739.1 NZ_CP027706:4916141-4916374 [Pseudomonas sp. CMR12a]
METGSVFKSNRSQAIRMPKAVALPDDVTRVDIVAIGRTRIITPAGESWDSWFDEQDSVSADFMTEREQPAEQVREEF

Nucleotide

• Length: 234 bp

>AT99894 NZ_CP027706:4916141-4916374 [Pseudomonas sp. CMR12a]
ATGGAAACCGGTTCCGTATTCAAAAGCAATCGCAGTCAGGCTATTCGCATGCCCAAGGCCGTGGCGTTGCCGGACGATGT
CACACGGGTGGACATCGTCGCGATCGGCCGCACCCGCATCATCACTCCGGCGGGCGAGTCCTGGGACAGTTGGTTTGATG
AGCAGGACAGCGTGAGCGCCGACTTCATGACTGAACGGGAGCAGCCAGCCGAACAGGTACGTGAGGAGTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G7A756