Detailed information of TA system TA099893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2817378..2818600
• Replicon: chromosome
• Accession: NZ_CP027706
• Organism: Pseudomonas sp. CMR12a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C4K39_RS13275
• Protein ID: WP_124346631.1
• Coordinates: 2818028..2818600 (+)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U6ZMH2
• Locus tag: C4K39_RS13270
• Protein ID: WP_022639877.1
• Coordinates: 2817378..2817935 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K39_RS13240	2812395..2812844	+	450	WP_124348344.1	ATP-binding protein	-
C4K39_RS13245	2812914..2813525	-	612	WP_068583852.1	hypothetical protein	-
C4K39_RS13250	2813796..2814215	+	420	WP_124346629.1	helix-turn-helix domain-containing protein	-
C4K39_RS13255	2814212..2815460	+	1249	Protein_2593	alpha/beta fold hydrolase	-
C4K39_RS13260	2815593..2816771	+	1179	WP_124346630.1	PrpF protein	-
C4K39_RS13265	2816877..2817206	-	330	WP_022639876.1	low molecular weight protein tyrosine phosphatase family protein	-
C4K39_RS13270	2817378..2817935	+	558	WP_022639877.1	helix-turn-helix domain-containing protein	Antitoxin
C4K39_RS13275	2818028..2818600	+	573	WP_124346631.1	HD domain-containing protein	Toxin
C4K39_RS13280	2818634..2819140	+	507	WP_068583868.1	N-acetyltransferase	-
C4K39_RS13285	2819152..2821494	-	2343	WP_124346632.1	penicillin-binding protein 1A	-
C4K39_RS13290	2821536..2823458	-	1923	WP_124346633.1	penicillin-binding protein 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (10-187)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-49)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21520.09 Da
• Isoelectric Point: 5.3042

>T99893 WP_124346631.1 NZ_CP027706:2818028-2818600 [Pseudomonas sp. CMR12a]
MNDQLLGGRLEFLRQAERLKDVLRSAHTSSGRQESTAEHSWRLCLMALLLEDQLGELDLLQVLKLCIVHDLGEALHGDIP
AVQQAAHPDKGQQEREDLQTLASCLDAPARQRLLALWDEYENASSAEAQAVKALDKLETLVQHNQGRNPADFDYRFNLDY
GRRYTSATPLFRALREQIDHDTRRHIDAAG

Nucleotide

• Length: 573 bp

>T99893 NZ_CP027706:2818028-2818600 [Pseudomonas sp. CMR12a]
ATGAACGATCAACTCCTCGGTGGTCGCCTCGAATTCCTGCGCCAGGCGGAGCGCCTCAAGGACGTACTGCGCAGTGCCCA
CACCTCCAGCGGTCGCCAGGAAAGCACCGCCGAGCACAGCTGGCGCCTGTGCCTGATGGCCTTGCTGCTGGAAGACCAGC
TGGGCGAGCTGGACCTTTTGCAAGTGCTCAAGCTGTGCATCGTCCACGACCTGGGCGAAGCCCTTCATGGCGACATCCCG
GCGGTGCAGCAGGCGGCCCACCCGGACAAGGGCCAGCAGGAACGCGAAGACCTGCAGACCCTCGCCAGTTGCCTCGATGC
CCCGGCCCGCCAGCGCCTGCTGGCCCTGTGGGACGAATACGAGAATGCCAGCTCGGCCGAGGCCCAGGCGGTCAAGGCCC
TGGATAAGCTGGAGACCCTGGTGCAGCACAACCAGGGGCGTAACCCGGCGGATTTCGATTACCGCTTCAACCTCGACTAC
GGCCGCCGCTACACTAGCGCCACGCCGCTGTTCCGAGCCCTGCGCGAGCAGATCGACCACGACACGCGGCGCCACATCGA
CGCGGCCGGCTGA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20413.32 Da
• Isoelectric Point: 7.0310

>AT99893 WP_022639877.1 NZ_CP027706:2817378-2817935 [Pseudomonas sp. CMR12a]
MSASLFTSPHLGLALRRWRLLHRVKQQHAAELFGVAQSSISRWENGQQAMEPSERAKLEQLLAANLSAAADQALARLVND
SPRAVHLVCDLTHRLLACSPTRAGEFGVPLSELLGQSLWGFCTEEIVRKERALDDLGWREVLAPPALEFFSGHNDSAIVP
IAASQCRWTRLNLSDGNAVRLVETL

Nucleotide

• Length: 558 bp

>AT99893 NZ_CP027706:2817378-2817935 [Pseudomonas sp. CMR12a]
ATGTCCGCATCCTTGTTCACTTCCCCGCACCTGGGCCTGGCCCTGCGCCGCTGGCGCCTGCTGCACCGGGTCAAGCAACA
GCACGCCGCCGAGCTGTTCGGGGTGGCGCAGTCGAGCATTTCCCGCTGGGAAAACGGCCAGCAAGCCATGGAACCCAGCG
AGCGCGCCAAGCTGGAGCAACTGCTGGCGGCCAACCTCAGCGCCGCCGCCGACCAGGCCCTGGCCCGGTTGGTGAACGAC
AGCCCGCGGGCGGTGCACCTGGTCTGCGACCTGACCCACCGCCTGCTGGCCTGCTCGCCTACCCGTGCCGGGGAGTTCGG
CGTGCCCTTGAGCGAGTTGCTGGGGCAATCCTTGTGGGGCTTTTGCACCGAGGAAATCGTGCGCAAGGAGAGGGCCCTCG
ACGATCTTGGCTGGCGCGAAGTGCTAGCGCCCCCGGCGCTGGAGTTCTTCAGCGGGCACAACGATTCGGCCATCGTGCCC
ATCGCGGCCAGCCAGTGCCGCTGGACCCGCCTGAACCTGTCCGACGGCAACGCGGTGCGGCTGGTGGAAACCCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G7A0D6