Detailed information of TA system TA099889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 710466..711062
• Replicon: chromosome
• Accession: NZ_CP027706
• Organism: Pseudomonas sp. CMR12a

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6G6ZTX3
• Locus tag: C4K39_RS03185
• Protein ID: WP_068577099.1
• Coordinates: 710760..711062 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4K39_RS03180
• Protein ID: WP_124345642.1
• Coordinates: 710466..710756 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K39_RS03160	706734..708866	-	2133	WP_124345640.1	TonB-dependent copper receptor	-
C4K39_RS03165	708955..709347	-	393	WP_124348311.1	DUF2946 domain-containing protein	-
C4K39_RS03170	709424..709903	-	480	WP_176719722.1	copper chaperone PCu(A)C	-
C4K39_RS03175	709968..710366	-	399	WP_124345641.1	DUF2946 domain-containing protein	-
C4K39_RS03180	710466..710756	-	291	WP_124345642.1	putative addiction module antidote protein	Antitoxin
C4K39_RS03185	710760..711062	-	303	WP_068577099.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K39_RS03190	711240..711968	-	729	WP_124345643.1	cobalt-precorrin-6A reductase	-
C4K39_RS03195	711965..713062	-	1098	WP_068577104.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
C4K39_RS03200	713055..714299	-	1245	WP_068577106.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
C4K39_RS03205	714348..715751	+	1404	WP_124345644.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-97)

Antitoxin

• Uncharacterized protein HI1420 (3-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.24 Da
• Isoelectric Point: 10.8593

>T99889 WP_068577099.1 NZ_CP027706:c711062-710760 [Pseudomonas sp. CMR12a]
MFVFEQTQEFSHWLTRLKDPQGKTRVLARIRAAQLGRFGDCVSIGGGVHEMRIHRGPGYRVYFSRRGERIYLLLIGGDKS
TQSRDIQRARQLAHDLKNED

Nucleotide

• Length: 303 bp

>T99889 NZ_CP027706:c711062-710760 [Pseudomonas sp. CMR12a]
ATGTTCGTCTTCGAGCAAACCCAGGAGTTCAGCCACTGGCTCACTCGCCTCAAGGACCCTCAGGGGAAGACCCGTGTACT
GGCCCGGATCAGGGCAGCGCAACTAGGGCGGTTTGGCGACTGTGTATCAATAGGAGGTGGAGTCCATGAGATGCGTATCC
ACCGTGGGCCTGGCTACCGGGTGTACTTTTCACGGCGAGGCGAACGGATCTACCTGTTGTTGATCGGTGGCGACAAGAGC
ACCCAGAGTCGCGACATCCAACGAGCCCGGCAACTGGCTCACGACCTCAAGAACGAGGACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10389.97 Da
• Isoelectric Point: 6.2732

>AT99889 WP_124345642.1 NZ_CP027706:c710756-710466 [Pseudomonas sp. CMR12a]
MRTPVTRFDAADYLKTPQDMVAYLDACFEEDAGDGVLIRAALNDIARARGMTQIARDTGLGRESLYKALGSQGNPEFATI
IKVMKALGLKLQVSPA

Nucleotide

• Length: 291 bp

>AT99889 NZ_CP027706:c710756-710466 [Pseudomonas sp. CMR12a]
ATGCGCACCCCAGTGACCCGCTTCGATGCGGCCGACTACCTCAAGACCCCGCAAGACATGGTGGCCTACCTCGATGCCTG
CTTCGAGGAAGATGCCGGCGATGGCGTGCTGATCCGCGCCGCCCTCAACGACATCGCCCGCGCCCGGGGCATGACCCAGA
TAGCCCGGGACACCGGCCTGGGCCGCGAAAGCCTGTACAAGGCGCTGGGCAGCCAGGGCAATCCCGAGTTCGCGACTATC
ATCAAGGTGATGAAGGCGCTGGGACTCAAGCTTCAGGTCAGCCCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A6G6ZTX3