Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 163469..164112 Replicon plasmid p675SK2_B
Accession NZ_CP027703
Organism Escherichia coli strain 675SK2

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag CYU58_RS26230 Protein ID WP_001034044.1
Coordinates 163696..164112 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag CYU58_RS26225 Protein ID WP_001261286.1
Coordinates 163469..163699 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYU58_RS26200 160100..160855 - 756 WP_000852146.1 replication initiation protein RepE -
CYU58_RS26205 161577..162383 - 807 WP_000016970.1 site-specific integrase -
CYU58_RS26210 162384..162689 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
CYU58_RS26215 162691..162909 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
CYU58_RS26225 163469..163699 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CYU58_RS26230 163696..164112 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
CYU58_RS26235 164187..165751 + 1565 Protein_196 AAA family ATPase -
CYU58_RS26240 165736..166757 + 1022 Protein_197 DNA helicase UvrD -
CYU58_RS26250 167011..167697 - 687 Protein_198 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / blaOXA-1 / aac(6')-Ib-cr / tet(B) / catA1 / aadA5 / qacE / sul1 / mph(A) / blaNDM-5 / aadA2 / dfrA12 iucB / iucA / iutA / iucD / iucC / iucB 1..173882 173882
- flank IS/Tn - - 167011..167358 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T99883 WP_001034044.1 NZ_CP027703:163696-164112 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T99883 NZ_CP027703:163696-164112 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT99883 WP_001261286.1 NZ_CP027703:163469-163699 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT99883 NZ_CP027703:163469-163699 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References