Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 162384..162909 Replicon plasmid p675SK2_B
Accession NZ_CP027703
Organism Escherichia coli strain 675SK2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CYU58_RS26210 Protein ID WP_001159871.1
Coordinates 162384..162689 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag CYU58_RS26215 Protein ID WP_000813630.1
Coordinates 162691..162909 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYU58_RS26195 158346..159512 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
CYU58_RS26200 160100..160855 - 756 WP_000852146.1 replication initiation protein RepE -
CYU58_RS26205 161577..162383 - 807 WP_000016970.1 site-specific integrase -
CYU58_RS26210 162384..162689 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CYU58_RS26215 162691..162909 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CYU58_RS26225 163469..163699 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CYU58_RS26230 163696..164112 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
CYU58_RS26235 164187..165751 + 1565 Protein_196 AAA family ATPase -
CYU58_RS26240 165736..166757 + 1022 Protein_197 DNA helicase UvrD -
CYU58_RS26250 167011..167697 - 687 Protein_198 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / blaOXA-1 / aac(6')-Ib-cr / tet(B) / catA1 / aadA5 / qacE / sul1 / mph(A) / blaNDM-5 / aadA2 / dfrA12 iucB / iucA / iutA / iucD / iucC / iucB 1..173882 173882
- flank IS/Tn - - 167011..167358 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T99882 WP_001159871.1 NZ_CP027703:c162689-162384 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T99882 NZ_CP027703:c162689-162384 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT99882 WP_000813630.1 NZ_CP027703:c162909-162691 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT99882 NZ_CP027703:c162909-162691 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References