Detailed information of TA system TA099857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2411942..2412580
• Replicon: chromosome
• Accession: NZ_CP027701
• Organism: Escherichia coli strain 675SK2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: CYU58_RS12445
• Protein ID: WP_001447010.1
• Coordinates: 2411942..2412118 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CYU58_RS12450
• Protein ID: WP_001270286.1
• Coordinates: 2412164..2412580 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYU58_RS12425	2407561..2408775	-	1215	WP_001326689.1	BenE family transporter YdcO	-
CYU58_RS12430	2408828..2409364	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
CYU58_RS12435	2409437..2411398	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CYU58_RS12440	2411490..2411720	-	231	WP_000494244.1	YncJ family protein	-
CYU58_RS12445	2411942..2412118	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CYU58_RS12450	2412164..2412580	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CYU58_RS12455	2412659..2414065	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
CYU58_RS12460	2414310..2415455	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
CYU58_RS12465	2415473..2416486	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
CYU58_RS12470	2416487..2417428	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T99857 WP_001447010.1 NZ_CP027701:2411942-2412118 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T99857 NZ_CP027701:2411942-2412118 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT99857 WP_001270286.1 NZ_CP027701:2412164-2412580 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT99857 NZ_CP027701:2412164-2412580 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8