Detailed information of TA system TA099853

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1124671..1125365
• Replicon: chromosome
• Accession: NZ_CP027701
• Organism: Escherichia coli strain 675SK2

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: CYU58_RS05755
• Protein ID: WP_001094398.1
• Coordinates: 1124997..1125365 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: CYU58_RS05750
• Protein ID: WP_077249034.1
• Coordinates: 1124671..1124976 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYU58_RS05705	1120623..1121078	+	456	WP_000581506.1	hypothetical protein	-
CYU58_RS05710	1121179..1121387	+	209	Protein_1026	DUF905 domain-containing protein	-
CYU58_RS05720	1121488..1122308	+	821	Protein_1027	DUF932 domain-containing protein	-
CYU58_RS05730	1122650..1123123	+	474	WP_000855059.1	antirestriction protein	-
CYU58_RS05735	1123139..1123615	+	477	WP_001424026.1	RadC family protein	-
CYU58_RS05740	1123684..1123905	+	222	WP_000692345.1	DUF987 domain-containing protein	-
CYU58_RS05745	1123924..1124567	+	644	Protein_1031	antitoxin of toxin-antitoxin stability system	-
CYU58_RS05750	1124671..1124976	+	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CYU58_RS05755	1124997..1125365	+	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
CYU58_RS05760	1125686..1126024	-	339	Protein_1034	LysR family transcriptional regulator	-
CYU58_RS05765	1126075..1127031	-	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
CYU58_RS05770	1127041..1129239	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
CYU58_RS05775	1129236..1130192	-	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1109019..1131910	22891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T99853 WP_001094398.1 NZ_CP027701:1124997-1125365 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T99853 NZ_CP027701:1124997-1125365 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT99853 WP_077249034.1 NZ_CP027701:1124671-1124976 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT99853 NZ_CP027701:1124671-1124976 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4