Detailed information of TA system TA099840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3275926..3276467
• Replicon: chromosome
• Accession: NZ_CP027669
• Organism: Simplicispira suum strain SC1-8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C6571_RS15175
• Protein ID: WP_106447428.1
• Coordinates: 3275926..3276252 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C6571_RS15180
• Protein ID: WP_106447429.1
• Coordinates: 3276249..3276467 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6571_RS15155	3271325..3273673	+	2349	WP_106447424.1	ATP-dependent Clp protease ATP-binding subunit ClpA	-
C6571_RS15160	3273919..3274629	-	711	WP_106447425.1	VIT family protein	-
C6571_RS15165	3274723..3275421	-	699	WP_106447426.1	RES family NAD+ phosphorylase	-
C6571_RS15170	3275426..3275797	-	372	WP_106447427.1	DUF2384 domain-containing protein	-
C6571_RS15175	3275926..3276252	-	327	WP_106447428.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C6571_RS15180	3276249..3276467	-	219	WP_106447429.1	antitoxin MazE family protein	Antitoxin
C6571_RS15185	3276607..3278058	+	1452	WP_106447430.1	exodeoxyribonuclease I	-
C6571_RS15190	3278061..3279377	+	1317	WP_106447431.1	protein kinase	-
C6571_RS15195	3279478..3281187	-	1710	WP_106447432.1	GDYXXLXY domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11640.67 Da
• Isoelectric Point: 8.0512

>T99840 WP_106447428.1 NZ_CP027669:c3276252-3275926 [Simplicispira suum]
MKRGDLVTIAMQGDFGKPRPALVIQADHFNEHATVTVLPVTSALVAAPLLRIPLQANAENGLQKPSQVMVDKTMTVKRDK
LGSVFGQIDSDAMVEVERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T99840 NZ_CP027669:c3276252-3275926 [Simplicispira suum]
ATGAAGCGCGGCGACTTGGTGACCATCGCGATGCAAGGCGATTTCGGCAAACCCAGACCCGCCTTGGTAATTCAGGCCGA
CCATTTCAACGAGCATGCCACCGTGACGGTGCTCCCAGTGACAAGCGCACTTGTCGCCGCCCCGTTGCTGCGCATCCCGC
TTCAAGCGAATGCCGAAAACGGTTTACAGAAGCCTTCACAGGTGATGGTGGACAAAACCATGACCGTGAAACGCGACAAG
CTGGGTTCGGTCTTCGGCCAAATCGATTCGGATGCCATGGTCGAGGTGGAGCGCTGCCTGGCCGTATTCCTGGGGATTGC
GAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8322.42 Da
• Isoelectric Point: 6.2049

>AT99840 WP_106447429.1 NZ_CP027669:c3276467-3276249 [Simplicispira suum]
MAHVNSRVQKHRDALRMAGLRLVQIWVPDTRRPDFAQECRRQSLLAAQADMADTDMQHFMNDALADLDGWTE

Nucleotide

• Length: 219 bp

>AT99840 NZ_CP027669:c3276467-3276249 [Simplicispira suum]
ATGGCGCATGTCAATTCACGGGTTCAAAAGCACCGCGACGCGTTGCGCATGGCGGGCCTTCGTTTGGTGCAAATATGGGT
GCCGGATACGAGGCGGCCTGATTTTGCGCAGGAATGCCGCCGCCAATCTCTTCTTGCCGCTCAGGCAGACATGGCAGATA
CGGACATGCAGCACTTCATGAATGACGCCTTGGCGGACTTGGATGGCTGGACAGAATGA