Detailed information of TA system TA099825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1724571..1725264
• Replicon: chromosome
• Accession: NZ_CP027667
• Organism: Melaminivora sp. SC2-9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6568_RS08250
• Protein ID: WP_106683688.1
• Coordinates: 1724836..1725264 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: C6568_RS08245
• Protein ID: WP_106683687.1
• Coordinates: 1724571..1724834 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6568_RS08220	1719772..1720905	+	1134	WP_106683682.1	type III-B CRISPR module-associated protein Cmr3	-
C6568_RS08225	1720925..1721818	+	894	WP_106683683.1	type III-B CRISPR module RAMP protein Cmr4	-
C6568_RS08230	1721823..1722188	+	366	WP_106683684.1	type III-B CRISPR module-associated protein Cmr5	-
C6568_RS08235	1722192..1723469	+	1278	WP_106683685.1	type III-B CRISPR module RAMP protein Cmr6	-
C6568_RS08240	1723483..1724475	+	993	WP_106683686.1	CRISPR system precrRNA processing endoribonuclease RAMP protein Cas6	-
C6568_RS08245	1724571..1724834	+	264	WP_106683687.1	antitoxin	Antitoxin
C6568_RS08250	1724836..1725264	+	429	WP_106683688.1	PIN domain-containing protein	Toxin
C6568_RS08255	1725441..1725746	+	306	WP_106683689.1	CRISPR-associated protein Csx16	-
C6568_RS08260	1725743..1726930	+	1188	WP_106683690.1	TIGR02221 family CRISPR-associated protein	-
C6568_RS08265	1726978..1728075	+	1098	WP_199792816.1	TIGR02584 family CRISPR-associated protein	-
C6568_RS08270	1728483..1728767	+	285	WP_106683692.1	CRISPR-associated endonuclease Cas2	-
C6568_RS08275	1728838..1729845	+	1008	WP_106683693.1	CRISPR-associated endonuclease Cas1	-
C6568_RS08280	1729914..1730216	+	303	WP_106683694.1	CRISPR-associated endonuclease Cas2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15796.07 Da
• Isoelectric Point: 8.0666

>T99825 WP_106683688.1 NZ_CP027667:1724836-1725264 [Melaminivora sp. SC2-9]
MRALLDVNVLIALHDRNHIHHGLAARWLHEHAEQGWASCPLTQNGCLRIMGQSGYANPQPLAVLTAMLARSTGTRFHQFW
PDDISLLDARHFDHSQIHGSRQLTDTYLLALALHHGGRLVTFDRRIAASAVPGAQPTHLVLL

Nucleotide

• Length: 429 bp

>T99825 NZ_CP027667:1724836-1725264 [Melaminivora sp. SC2-9]
ATGCGGGCGCTGCTCGATGTCAACGTGTTGATTGCGCTGCACGACCGCAACCACATCCATCACGGCCTTGCAGCGCGCTG
GCTGCACGAGCACGCCGAACAGGGCTGGGCCAGTTGTCCGCTGACGCAAAACGGCTGCCTGCGCATCATGGGCCAGTCCG
GCTACGCCAACCCGCAGCCGCTGGCCGTGCTGACGGCCATGCTGGCCCGATCGACAGGCACGCGCTTTCACCAGTTCTGG
CCTGACGACATCAGCTTGCTCGATGCCCGGCACTTCGACCACAGCCAGATCCACGGCTCACGGCAACTGACGGACACCTA
CCTGCTGGCCCTTGCGTTGCACCACGGCGGGCGGCTGGTCACTTTTGACCGGCGCATCGCGGCCAGCGCCGTTCCTGGCG
CGCAGCCGACGCATCTGGTCTTGCTCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9269.66 Da
• Isoelectric Point: 6.4569

>AT99825 WP_106683687.1 NZ_CP027667:1724571-1724834 [Melaminivora sp. SC2-9]
MRTTLDIAEDVLLAAKELARRDKKPLGQVISELARKAFTLPDSPAARPGAGQNPRDPLAALGIHPLPPCGVVVSNAHIDQ
LRDEEGV

Nucleotide

• Length: 264 bp

>AT99825 NZ_CP027667:1724571-1724834 [Melaminivora sp. SC2-9]
ATGCGCACCACGCTCGATATTGCAGAGGATGTGCTGCTGGCCGCCAAGGAGCTGGCGCGGCGCGACAAAAAGCCTTTGGG
ACAGGTCATCAGCGAATTGGCGCGCAAGGCCTTTACCTTGCCGGACAGCCCCGCCGCGCGGCCAGGCGCCGGGCAAAACC
CGCGCGACCCGCTGGCGGCGCTGGGCATTCACCCCCTGCCGCCGTGCGGCGTGGTCGTCAGCAACGCCCACATCGACCAG
TTGCGCGACGAGGAAGGCGTCTGA