Detailed information of TA system TA099800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1535740..1536362
• Replicon: chromosome
• Accession: NZ_CP027656
• Organism: Pseudomonas chlororaphis subsp. piscium strain ZJU60

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C6Q18_RS06955
• Protein ID: WP_106696706.1
• Coordinates: 1536180..1536362 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C6Q18_RS06950
• Protein ID: WP_106698998.1
• Coordinates: 1535740..1536147 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6Q18_RS06930	1531042..1531935	+	894	WP_199796876.1	LysR family transcriptional regulator	-
C6Q18_RS06935	1532171..1533949	+	1779	WP_106696703.1	GGDEF and EAL domain-containing protein	-
C6Q18_RS06940	1533983..1534897	-	915	WP_106696704.1	SGNH/GDSL hydrolase family protein	-
C6Q18_RS06945	1535027..1535494	-	468	WP_106696705.1	GAF domain-containing protein	-
C6Q18_RS06950	1535740..1536147	-	408	WP_106698998.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C6Q18_RS06955	1536180..1536362	-	183	WP_106696706.1	type II toxin-antitoxin system HicA family toxin	Toxin
C6Q18_RS06965	1537081..1537965	-	885	WP_009047472.1	alpha/beta hydrolase	-
C6Q18_RS06970	1538317..1539009	-	693	WP_025804997.1	16S rRNA pseudouridine(516) synthase	-
C6Q18_RS06975	1539043..1539276	-	234	WP_106696707.1	cysteine-rich CWC family protein	-
C6Q18_RS06980	1539269..1540756	-	1488	WP_106696708.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6810.12 Da
• Isoelectric Point: 11.6709

>T99800 WP_106696706.1 NZ_CP027656:c1536362-1536180 [Pseudomonas chlororaphis subsp. piscium]
VNSRYLIGQIVADGWYLVRVRGSHHPFRHPSKPGLVTVPHPKKDLLRKTAISILKQALLM

Nucleotide

• Length: 183 bp

>T99800 NZ_CP027656:c1536362-1536180 [Pseudomonas chlororaphis subsp. piscium]
GTGAACAGTCGGTATTTGATCGGCCAAATCGTCGCAGACGGTTGGTATCTGGTCAGGGTCAGAGGCAGTCACCACCCTTT
CAGGCACCCCAGTAAACCGGGGCTGGTGACAGTTCCACATCCCAAAAAGGACCTGCTGAGAAAAACCGCCATCAGCATTC
TGAAACAGGCGCTGCTCATGTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14657.65 Da
• Isoelectric Point: 4.4710

>AT99800 WP_106698998.1 NZ_CP027656:c1536147-1535740 [Pseudomonas chlororaphis subsp. piscium]
MLYPIAISMGDDEHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFEILAEDGAAIPPANKVTLHAANPQYAGRTWAL
IDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEAKSRSGFLASAALKVLQQD

Nucleotide

• Length: 408 bp

>AT99800 NZ_CP027656:c1536147-1535740 [Pseudomonas chlororaphis subsp. piscium]
ATGCTTTACCCGATTGCAATTTCAATGGGCGATGACGAACACGCCTGGGGCGTGGAAGTCCCCGACATTCCTGGCTGTTT
CTCGGCCGGCGACGACCTCGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATTCTCGCCGAAG
ACGGTGCCGCCATCCCGCCCGCCAACAAGGTCACCCTGCACGCGGCCAACCCTCAGTACGCAGGCCGCACCTGGGCGCTG
ATCGACATCGATGTCACCAAGTACCTGGGCAAGGCGCAGAAGCTCAACATCACCCTGCCCGGCTACCTGCTGAACCGCAT
CGACGAATACGTGCTGCATCACCCGGAAGCAAAAAGCCGCTCGGGTTTTCTCGCTTCGGCTGCATTGAAGGTATTGCAGC
AAGACTGA