Detailed information of TA system TA099795

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 92338..92897
• Replicon: chromosome
• Accession: NZ_CP027643
• Organism: Brucella canis strain Brucella.canis isolate Jia bo Ding

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: C6Y57_RS06215
• Protein ID: WP_004688321.1
• Coordinates: 92338..92583 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: C6Y57_RS06220
• Protein ID: WP_002964097.1
• Coordinates: 92583..92897 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6Y57_RS06195	88184..89830	-	1647	WP_004689673.1	hypothetical protein	-
C6Y57_RS06200	89965..90738	-	774	WP_006132649.1	hypothetical protein	-
C6Y57_RS06205	90739..91497	-	759	Protein_76	IS5-like element IS711 family transposase	-
C6Y57_RS06210	91567..92205	-	639	Protein_77	tRNA-guanine transglycosylase	-
C6Y57_RS06215	92338..92583	+	246	WP_004688321.1	BrnT family toxin	Toxin
C6Y57_RS06220	92583..92897	+	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
C6Y57_RS06230	93245..93415	+	171	WP_002964095.1	DUF1508 domain-containing protein	-
C6Y57_RS06235	93763..94473	-	711	Protein_81	porin family protein	-
C6Y57_RS06240	94708..95295	-	588	WP_004683738.1	hypothetical protein	-
C6Y57_RS06245	95318..95593	-	276	WP_002964092.1	hypothetical protein	-
C6Y57_RS06250	95590..95820	-	231	WP_002964091.1	hypothetical protein	-
C6Y57_RS06255	95817..96536	-	720	WP_006072696.1	hypothetical protein	-
C6Y57_RS06260	96586..96789	+	204	WP_002964088.1	hypothetical protein	-
C6Y57_RS06265	96786..97001	+	216	WP_002971459.1	hypothetical protein	-
C6Y57_RS06270	97004..97207	+	204	WP_004690812.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	90739..91011	272

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T99795 WP_004688321.1 NZ_CP027643:92338-92583 [Brucella canis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T99795 NZ_CP027643:92338-92583 [Brucella canis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT99795 WP_002964097.1 NZ_CP027643:92583-92897 [Brucella canis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT99795 NZ_CP027643:92583-92897 [Brucella canis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA