Detailed information of TA system TA099794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 23262..24180
• Replicon: plasmid unnamed1
• Accession: NZ_CP027635
• Organism: Campylobacter coli strain meC0281

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: -
• Locus tag: DSM104626_RS09295
• Protein ID: WP_072240469.1
• Coordinates: 23497..24180 (+)
• Length: 228 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: -
• Locus tag: DSM104626_RS09290
• Protein ID: WP_057098774.1
• Coordinates: 23262..23504 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM104626_RS09275	18396..20609	+	2214	WP_200408900.1	DUF1738 domain-containing protein	-
DSM104626_RS09280	20606..22429	+	1824	WP_200408901.1	type IV secretory system conjugative DNA transfer family protein	-
DSM104626_RS09285	22440..23159	+	720	WP_002844665.1	conjugal transfer protein TraL	-
DSM104626_RS09290	23262..23504	+	243	WP_057098774.1	hypothetical protein	Antitoxin
DSM104626_RS09295	23497..24180	+	684	WP_072240469.1	Fic family protein	Toxin
DSM104626_RS09300	24185..24652	+	468	WP_002814358.1	signal peptidase I	-
DSM104626_RS09305	24717..25112	+	396	WP_200408902.1	conjugal transfer protein TraM	-
DSM104626_RS09310	25204..25890	+	687	WP_002823899.1	AAA family ATPase	-
DSM104626_RS09315	25893..26117	+	225	WP_002846381.1	hypothetical protein	-
DSM104626_RS09320	26259..26732	+	474	WP_002817206.1	IS200/IS605 family transposase	-
DSM104626_RS09325	26719..27957	+	1239	WP_002784330.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30719	30719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (60-169)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 228 a.a.
• Molecular weight: 26184.38 Da
• Isoelectric Point: 10.1130

>T99794 WP_072240469.1 NZ_CP027635:23497-24180 [Campylobacter coli]
MIDKVLKFLDDGIPKNDFDRQLLASNLVGATNLIELNKREREITLKKQLKLQLNPIKGNFDYQHLKDIHKALFEDVYTWA
GQDRMQMGLKEKFAKYAPNGAIINFVPGKELDKYSKIIFDELAKNNYLKNSKDLNHFAKNLAKFMGEINALHPFREGNGR
TQRIFLNELAKNAGYKLDLNLISKHKMIHACVEASQLKPGRLEALIKDNLKNFRQNLDLEQNKGMSL

Nucleotide

• Length: 684 bp

>T99794 NZ_CP027635:23497-24180 [Campylobacter coli]
ATGATTGATAAAGTTTTGAAATTTTTAGATGATGGTATTCCTAAAAATGATTTTGACAGACAACTTTTAGCTTCTAATTT
AGTAGGCGCAACAAATTTAATAGAGTTAAATAAACGAGAAAGAGAAATTACATTAAAAAAACAATTAAAACTACAACTCA
ATCCCATTAAAGGCAATTTCGATTATCAGCATTTAAAAGATATACATAAAGCACTTTTTGAAGATGTTTATACTTGGGCA
GGACAAGATAGAATGCAAATGGGACTAAAAGAAAAATTTGCAAAATATGCACCCAATGGAGCAATTATTAATTTTGTTCC
TGGAAAAGAGTTAGATAAATACTCTAAAATAATTTTTGATGAACTTGCAAAAAATAACTATCTAAAAAACTCCAAAGATC
TAAATCATTTTGCAAAAAATTTAGCAAAATTTATGGGCGAAATCAATGCTTTACACCCTTTTAGAGAAGGTAACGGCAGA
ACTCAAAGAATATTTTTAAATGAATTAGCTAAAAATGCAGGTTATAAACTTGATTTAAATTTAATTTCTAAGCATAAAAT
GATACATGCTTGCGTAGAAGCTTCACAATTAAAACCTGGAAGATTGGAGGCTTTAATTAAAGACAATCTAAAAAACTTTA
GACAAAATTTAGATTTGGAGCAAAATAAAGGAATGTCGTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9276.53 Da
• Isoelectric Point: 4.4798

>AT99794 WP_057098774.1 NZ_CP027635:23262-23504 [Campylobacter coli]
MQKKYPEVHSLEESLAILKKYKDDLTKEQYEGIKSVICGHAIEDMFANEKDVIDMIKIAKDEANADEIIAEYKREWGVND

Nucleotide

• Length: 243 bp

>AT99794 NZ_CP027635:23262-23504 [Campylobacter coli]
ATGCAAAAAAAATATCCCGAAGTGCATAGCTTAGAAGAAAGCCTTGCGATACTTAAAAAATATAAAGATGATTTAACTAA
AGAGCAATATGAAGGTATTAAATCTGTTATTTGCGGACACGCTATTGAAGATATGTTTGCAAATGAAAAAGATGTTATCG
ATATGATAAAAATTGCAAAAGATGAAGCAAATGCCGATGAAATAATCGCCGAATATAAAAGAGAATGGGGAGTAAATGAT
TGA