Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2688107..2688727 Replicon chromosome
Accession NZ_CP027618
Organism Enterobacter cloacae complex sp. FDA-CDC-AR_0132

Toxin (Protein)


Gene name Hha Uniprot ID G8LPY2
Locus tag AM379_RS12990 Protein ID WP_014168902.1
Coordinates 2688107..2688325 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID W0BRV6
Locus tag AM379_RS12995 Protein ID WP_020885187.1
Coordinates 2688353..2688727 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM379_RS12960 2684118..2684378 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
AM379_RS12965 2684381..2684521 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM379_RS12970 2684518..2685228 - 711 WP_020885191.1 GNAT family N-acetyltransferase -
AM379_RS12975 2685329..2686789 + 1461 WP_106503773.1 PLP-dependent aminotransferase family protein -
AM379_RS12980 2686761..2687228 - 468 WP_020885189.1 extracytoplasmic function sigma factor YlaC -
AM379_RS12985 2687346..2687897 - 552 WP_020885188.1 maltose O-acetyltransferase -
AM379_RS12990 2688107..2688325 - 219 WP_014168902.1 hemolysin expression modulator Hha Toxin
AM379_RS12995 2688353..2688727 - 375 WP_020885187.1 Hha toxicity modulator TomB Antitoxin
AM379_RS13000 2689239..2692385 - 3147 WP_014168904.1 multidrug efflux RND transporter permease subunit -
AM379_RS13005 2692408..2693601 - 1194 WP_020885186.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8654.04 Da        Isoelectric Point: 8.9107

>T99785 WP_014168902.1 NZ_CP027618:c2688325-2688107 [Enterobacter cloacae complex sp. FDA-CDC-AR_0132]
MSEKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWRFVR

Download         Length: 219 bp

>T99785 NZ_CP027618:c2688325-2688107 [Enterobacter cloacae complex sp. FDA-CDC-AR_0132]
ATGTCTGAAAAACCACTCACAAAAATCGATTATTTGATGCGTTTGCGACGCTGTCAGTCAATAGACACGCTTGAACGTGT
AATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCGGCTGACCACCGTCTTGCTGAAC
TGACCATGAATAAGCTGTATGACAAAATCCCAACGTCCGTGTGGAGATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8886

>AT99785 WP_020885187.1 NZ_CP027618:c2688727-2688353 [Enterobacter cloacae complex sp. FDA-CDC-AR_0132]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFINVSRANPVSLSC

Download         Length: 375 bp

>AT99785 NZ_CP027618:c2688727-2688353 [Enterobacter cloacae complex sp. FDA-CDC-AR_0132]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTAAACGAGTTGATCGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGATGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAATGCGCAAGATTTGCAGAAATGGCGTAAATCGGGCAACCGACT
ATTTCGTTGCTTCATCAACGTGAGCAGAGCTAACCCCGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A839BM33

Antitoxin

Source ID Structure
AlphaFold DB A0A5C1BX74

References