Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 43912..44555 Replicon plasmid unnamed4
Accession NZ_CP027616
Organism Klebsiella pneumoniae subsp. ozaenae strain AR_0096

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AM475_RS28535 Protein ID WP_001044770.1
Coordinates 44139..44555 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AM475_RS28530 Protein ID WP_001261282.1
Coordinates 43912..44142 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM475_RS28500 39629..40384 - 756 WP_012539983.1 replication initiation protein RepE -
AM475_RS30200 41015..41128 + 114 WP_032072094.1 hypothetical protein -
AM475_RS30205 41257..41514 + 258 WP_032408936.1 hypothetical protein -
AM475_RS28515 41572..42357 - 786 WP_022644886.1 site-specific integrase -
AM475_RS28520 42360..42998 - 639 WP_022644887.1 hypothetical protein -
AM475_RS29680 43047..43367 - 321 WP_032072060.1 hypothetical protein -
AM475_RS30210 43495..43955 - 461 Protein_55 hypothetical protein -
AM475_RS28530 43912..44142 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM475_RS28535 44139..44555 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AM475_RS28540 44629..44865 + 237 WP_001348075.1 AAA family ATPase -
AM475_RS28545 44912..45616 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
AM475_RS28550 45669..46436 - 768 Protein_60 class 1 integron integrase IntI1 -
AM475_RS28555 46592..47065 + 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
AM475_RS28560 47286..47552 + 267 WP_001144737.1 plasmid mobilization relaxosome protein MobC -
AM475_RS28570 47695..48459 + 765 WP_001389365.1 IS6-like element IS6100 family transposase -
AM475_RS30215 48599..48664 - 66 Protein_64 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaKPC-3 - 1..129985 129985


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T99783 WP_001044770.1 NZ_CP027616:44139-44555 [Klebsiella pneumoniae subsp. ozaenae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T99783 NZ_CP027616:44139-44555 [Klebsiella pneumoniae subsp. ozaenae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT99783 WP_001261282.1 NZ_CP027616:43912-44142 [Klebsiella pneumoniae subsp. ozaenae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT99783 NZ_CP027616:43912-44142 [Klebsiella pneumoniae subsp. ozaenae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References