Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3453947..3454566 Replicon chromosome
Accession NZ_CP027612
Organism Klebsiella pneumoniae subsp. ozaenae strain AR_0096

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AM475_RS17360 Protein ID WP_002892050.1
Coordinates 3453947..3454165 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag AM475_RS17365 Protein ID WP_002892066.1
Coordinates 3454192..3454566 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM475_RS17330 3449994..3450257 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
AM475_RS17335 3450257..3450397 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
AM475_RS17340 3450394..3451092 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
AM475_RS17345 3451193..3452644 + 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
AM475_RS17350 3452619..3453089 - 471 WP_002892026.1 YlaC family protein -
AM475_RS29980 3453110..3453250 + 141 WP_004147370.1 hypothetical protein -
AM475_RS17355 3453222..3453788 - 567 WP_002892030.1 maltose O-acetyltransferase -
AM475_RS17360 3453947..3454165 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AM475_RS17365 3454192..3454566 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
AM475_RS17370 3455052..3458198 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
AM475_RS17375 3458221..3459414 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T99781 WP_002892050.1 NZ_CP027612:c3454165-3453947 [Klebsiella pneumoniae subsp. ozaenae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T99781 NZ_CP027612:c3454165-3453947 [Klebsiella pneumoniae subsp. ozaenae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT99781 WP_002892066.1 NZ_CP027612:c3454566-3454192 [Klebsiella pneumoniae subsp. ozaenae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT99781 NZ_CP027612:c3454566-3454192 [Klebsiella pneumoniae subsp. ozaenae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References