Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2250295..2250920 Replicon chromosome
Accession NZ_CP027612
Organism Klebsiella pneumoniae subsp. ozaenae strain AR_0096

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag AM475_RS11385 Protein ID WP_004187928.1
Coordinates 2250537..2250920 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AM475_RS11380 Protein ID WP_004150355.1
Coordinates 2250295..2250537 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM475_RS11355 2246297..2246896 + 600 WP_004151865.1 glucose-1-phosphatase -
AM475_RS11360 2246890..2247750 + 861 WP_004146232.1 virulence factor BrkB family protein -
AM475_RS11365 2247747..2248184 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AM475_RS11370 2248229..2249170 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AM475_RS11375 2249184..2250101 - 918 WP_004187929.1 alpha/beta hydrolase -
AM475_RS11380 2250295..2250537 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AM475_RS11385 2250537..2250920 + 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
AM475_RS11390 2251094..2252023 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AM475_RS11395 2252020..2252655 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
AM475_RS11400 2252652..2253554 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T99776 WP_004187928.1 NZ_CP027612:2250537-2250920 [Klebsiella pneumoniae subsp. ozaenae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T99776 NZ_CP027612:2250537-2250920 [Klebsiella pneumoniae subsp. ozaenae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT99776 WP_004150355.1 NZ_CP027612:2250295-2250537 [Klebsiella pneumoniae subsp. ozaenae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT99776 NZ_CP027612:2250295-2250537 [Klebsiella pneumoniae subsp. ozaenae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTACTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References