Detailed information of TA system TA099775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1846527..1847113
• Replicon: chromosome
• Accession: NZ_CP027612
• Organism: Klebsiella pneumoniae subsp. ozaenae strain AR_0096

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: AM475_RS09425
• Protein ID: WP_002920800.1
• Coordinates: 1846527..1846895 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: AM475_RS09430
• Protein ID: WP_004174006.1
• Coordinates: 1846892..1847113 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM475_RS09405	1842030..1843100	-	1071	WP_004188298.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
AM475_RS09410	1843102..1843947	-	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
AM475_RS09415	1843944..1844831	-	888	WP_014906831.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
AM475_RS09420	1844938..1846254	-	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
AM475_RS09425	1846527..1846895	-	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AM475_RS09430	1846892..1847113	-	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AM475_RS09435	1847277..1847990	-	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
AM475_RS09440	1847992..1848759	-	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
AM475_RS09445	1848756..1850033	-	1278	WP_004188292.1	branched chain amino acid ABC transporter permease LivM	-
AM475_RS09450	1850030..1850956	-	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1841293..1886897	45604

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T99775 WP_002920800.1 NZ_CP027612:c1846895-1846527 [Klebsiella pneumoniae subsp. ozaenae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T99775 NZ_CP027612:c1846895-1846527 [Klebsiella pneumoniae subsp. ozaenae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT99775 WP_004174006.1 NZ_CP027612:c1847113-1846892 [Klebsiella pneumoniae subsp. ozaenae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT99775 NZ_CP027612:c1847113-1846892 [Klebsiella pneumoniae subsp. ozaenae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7