Detailed information of TA system TA099759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 4376..5173
• Replicon: plasmid unnamed1
• Accession: NZ_CP027605
• Organism: Enterobacter cloacae complex sp. strain AR_0093

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: AM472_RS23435
• Protein ID: WP_172754447.1
• Coordinates: 4376..4879 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: AM472_RS23440
• Protein ID: WP_016582626.1
• Coordinates: 4904..5173 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM472_RS23415 (AM472_23395)	2046..2834	+	789	WP_040110304.1	receptor-recognizing protein	-
AM472_RS23420 (AM472_23400)	2909..3232	+	324	WP_000064173.1	hypothetical protein	-
AM472_RS23425 (AM472_23405)	3246..3938	+	693	WP_006812500.1	membrane protein	-
AM472_RS24865	3940..4191	+	252	WP_040110303.1	hypothetical protein	-
AM472_RS24870	4184..4348	+	165	WP_228100666.1	hypothetical protein	-
AM472_RS23435 (AM472_23415)	4376..4879	-	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
AM472_RS23440 (AM472_23420)	4904..5173	-	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
AM472_RS23445 (AM472_23425)	5590..6255	+	666	WP_023315962.1	AAA family ATPase	-
AM472_RS23450 (AM472_23430)	6261..6614	+	354	WP_160956455.1	hypothetical protein	-
AM472_RS23455 (AM472_23435)	6656..7453	-	798	WP_077990606.1	DUF2971 domain-containing protein	-
AM472_RS23460 (AM472_23440)	7717..8442	+	726	WP_006812583.1	hypothetical protein	-
AM472_RS23465 (AM472_23445)	8503..8856	+	354	WP_219859204.1	hypothetical protein	-
AM472_RS23470 (AM472_23450)	8995..9855	-	861	WP_000027057.1	broad-spectrum class A beta-lactamase TEM-1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B	-	1..118226	118226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T99759 WP_172754447.1 NZ_CP027605:c4879-4376 [Enterobacter cloacae complex sp.]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T99759 NZ_CP027605:c4879-4376 [Enterobacter cloacae complex sp.]
ATGTTTTCAGCACAAGCCGTATACGATTTTTCGGGCTTCGACTGCGGCGAACCATCTCTGAATGATTTCCTGCAAAACCG
CCTGGTACAGCAACACAACGGGCGTATATTACGTGGCTACCTGCTTCTTACCAAAGATCCGGTGCCGAAAGTTAAGGGTT
TTTACACGCTATCCGGGAGCTGTTTTGCAAGACAGACGCTACCATCGAACACCCAGAAGCGGAAAATCCCCTACGCGGAT
GCCCCCAGCGTTACGCTTGGCCGTCTGGCAATAGATATCAGCCTACAGAAACAAGGCTACGGGGAGGTTCTCGTAACGCA
CGCGTTGAAGGTTGTTTATCAGGCTTCTCGCGCAGTAGGCATATACGCTTTGTTTGTGGATGCACTAAACCAAAGCGCAA
TGCAGTTCTATCAGAAGTTTGGATTCATCCCTTTGACGGGGGCAAATGCCAACTCGCTTTTCTACCCCACGAAAAGCATT
GAAGAGCTGTTTGAAACAGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT99759 WP_016582626.1 NZ_CP027605:c5173-4904 [Enterobacter cloacae complex sp.]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT99759 NZ_CP027605:c5173-4904 [Enterobacter cloacae complex sp.]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACGATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGCCGCT
TGATTCTCAGTGAAGAGTCCTGGAATCTGGTAATGGATGCCATCAGTAACCCGCCTGCACCGAACGATAGGCTGAAACGA
GCTGCCAAACGTCTACAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3