Detailed information of TA system TA099757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4422338..4422943
• Replicon: chromosome
• Accession: NZ_CP027604
• Organism: Enterobacter cloacae complex sp. strain AR_0093

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: AM472_RS21670
• Protein ID: WP_012906750.1
• Coordinates: 4422338..4422517 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A822X3T2
• Locus tag: AM472_RS21675
• Protein ID: WP_032619507.1
• Coordinates: 4422539..4422943 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM472_RS21635 (AM472_21615)	4417549..4417986	-	438	WP_003863117.1	type II toxin-antitoxin system RatA family toxin	-
AM472_RS21640 (AM472_21620)	4418143..4418625	+	483	WP_003863115.1	SsrA-binding protein SmpB	-
AM472_RS21650 (AM472_21630)	4419368..4420573	+	1206	WP_039269032.1	DUF4236 domain-containing protein	-
AM472_RS21655 (AM472_21635)	4420829..4421500	+	672	WP_039269186.1	SOS response-associated peptidase	-
AM472_RS21660 (AM472_21640)	4421510..4421827	-	318	WP_039269033.1	hypothetical protein	-
AM472_RS25140 (AM472_21645)	4421827..4422066	-	240	WP_039269187.1	hypothetical protein	-
AM472_RS21670 (AM472_21650)	4422338..4422517	+	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM472_RS21675 (AM472_21655)	4422539..4422943	+	405	WP_032619507.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM472_RS21680 (AM472_21660)	4422984..4424054	-	1071	WP_039269035.1	glycosyltransferase family 9 protein	-
AM472_RS21685 (AM472_21665)	4424131..4424709	-	579	WP_039269037.1	tail fiber assembly protein	-
AM472_RS21690 (AM472_21670)	4424709..4426886	-	2178	WP_052255160.1	phage tail protein	-
AM472_RS21695 (AM472_21675)	4426889..4427440	-	552	WP_032619504.1	phage tail protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4420829..4462324	41495

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T99757 WP_012906750.1 NZ_CP027604:4422338-4422517 [Enterobacter cloacae complex sp.]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T99757 NZ_CP027604:4422338-4422517 [Enterobacter cloacae complex sp.]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTTAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCGACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14676.53 Da
• Isoelectric Point: 4.2136

>AT99757 WP_032619507.1 NZ_CP027604:4422539-4422943 [Enterobacter cloacae complex sp.]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGEDYQDAIESVREAIEAHIELLVEDGESVPEATTVENWLSDPEYAGAVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVINQR

Nucleotide

• Length: 405 bp

>AT99757 NZ_CP027604:4422539-4422943 [Enterobacter cloacae complex sp.]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGAAGATTATCAGGACGCGATCGAAAGCGTACGTGAAGCGATCGAGGCACACATCGAATTACTGGTTGAAGATGGCG
AGAGCGTTCCCGAAGCAACGACCGTCGAAAACTGGCTATCTGATCCGGAGTACGCCGGTGCGGTATGGGCCCTAGTTGAC
GTGGATATAACACGCCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTACCCTCATTACTGATCCGACGTATTGATCA
GTTTGTGGCCGCGCATCCTGAATATGGAAGCCGTTCGGGCTTCCTTTCTCGCGTCGCTGCTGACAAGGTTATTAATCAAC
GATAG

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4

Antitoxin

• AlphaFold DB: A0A822X3T2