99756

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA(toxin)
Location 4028363..4030146 Replicon chromosome
Accession NZ_CP027604
Organism Enterobacter cloacae complex sp. strain AR_0093

Toxin (Protein)


Gene name hipA Uniprot ID A0A8B5ZXW9
Locus tag AM472_RS19780 Protein ID WP_022651516.1
Coordinates 4028363..4029712 (+) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A837FFV9
Locus tag AM472_RS19785 Protein ID WP_003859317.1
Coordinates 4029709..4030146 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM472_RS19755 (AM472_19735) 4024186..4025121 - 936 WP_003859306.1 tRNA dihydrouridine(16) synthase DusC -
AM472_RS19760 (AM472_19740) 4025290..4025685 + 396 WP_003859308.1 CidA/LrgA family protein -
AM472_RS19765 (AM472_19745) 4025682..4026377 + 696 WP_003859310.1 CidB/LrgB family autolysis modulator -
AM472_RS19770 (AM472_19750) 4026506..4027390 + 885 WP_022651515.1 cytidine deaminase -
AM472_RS19775 (AM472_19755) 4027518..4028228 + 711 WP_003859314.1 outer membrane permeability protein SanA -
AM472_RS19780 (AM472_19760) 4028363..4029712 + 1350 WP_022651516.1 HipA domain-containing protein Toxin
AM472_RS19785 (AM472_19765) 4029709..4030146 + 438 WP_003859317.1 helix-turn-helix transcriptional regulator Antitoxin
AM472_RS19790 (AM472_19770) 4030185..4031195 - 1011 WP_003859318.1 galactose/methyl galactoside ABC transporter permease MglC -
AM472_RS19795 (AM472_19775) 4031211..4032731 - 1521 WP_039268972.1 galactose/methyl galactoside ABC transporter ATP-binding protein MglA -
AM472_RS19800 (AM472_19780) 4032811..4033809 - 999 WP_003859322.1 galactose/glucose ABC transporter substrate-binding protein MglB -
AM472_RS19805 (AM472_19785) 4034105..4035127 - 1023 WP_003859324.1 HTH-type transcriptional regulator GalS -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-406)

Antitoxin

Cro/C1-type helix-turn-helix domain

(75-128)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51151.85 Da        Isoelectric Point: 4.7049

>T99756 WP_022651516.1 NZ_CP027604:4028363-4029712 [Enterobacter cloacae complex sp.]
METLTVQAYLNETWTDIALIKYPGSEKKDWNTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Download         Length: 1350 bp

>T99756 NZ_CP027604:4028363-4029712 [Enterobacter cloacae complex sp.]
ATGGAGACGTTAACCGTACAGGCATATCTGAACGAAACATGGACGGATATCGCTCTCATCAAATATCCTGGAAGTGAAAA
AAAAGACTGGAATACCACGCAGTTAGACTATCTTACTGAGTATGCGATTAATTTTCTCGACTATGATGATTTTCACGCAG
TCTCAGTCAATCACCCTGTATCACTTTTTTTTGATGACCATGGCCAACCGGGCTGGCTGCGATTTATCGACGATATCATT
CCCGCCGGAGCAAGCCGCCGTTACTGGATTAATGCGCTCGACATCAGTGAGTTACCCGTTGGCCAGCAAAATTTCTTATT
GCTGAAATTTGGCACAATGTCCCCTGTGGGGAATTTGCGCATAAAGGAATCCGTCCCAGAATGGAATAAATTCGCCAGCA
CTAAAACGTTTACGGTTGCGGACGTCATCGATCGTGCGGCCGATTTTCTGGATTATGCCCAGGAAAGAGGCGCGGCGGCG
GGTGGTGCAACCGGTGCTGGCGGTGAAGCGCCTAAACTTTTGCTGCGCTGTAGTGAAACTGATGCCATCTGGATCGATAC
CTGGCAAAACGAGCCGGATAACCGGGACCGCTATTATTTGGTTAAATATCCACGCGGTAACAGGACAGAAGTGGATTGTA
ATATTCTGCGAGCTGAATTCCATTATTATCATGAGCTTAACGCGATGGGTTTTTCCACGATCCCAGTGGAAACAATGCGC
CTTGAAGAAGGTAGCCATTACCCGTCTCTGTGGTTACCGCGTTTTGACATAGTGAGAAATGAGCAGGACCAACTGGTCAG
GCTGGGTATGGAATCGGTGTATTCGCTATTACAAAAGGCACCCGGCACCCTCCTTGATCATGAGACAACGTTGCGCGCGT
TAATTAATAAAATCACCTCCAGCCATATGGTCCAGCAGCAGGGTTATCATTTTGATATTCCGGGATTTGTAACAGAATGG
GTGCGTCGCGATCTGCTAAATATTATTTTTGGAAATAGTGACAACCATGGGCGAAATACCGCCTTTGTGAAGGCTGAAAA
TCAAATTATGCTCTCCCCTGTATATGACTTTGCGCCGATGAAAGCCGATCCTGAAGGGATACCAAGAACCCTGAAGTGGT
CATTATCTTGCGAATCAGGGGGGGATTATAATTTCAACACTATCGCACAAACGCTGGCGGAGTGGATCCCTCCCGCAACA
TTACTTGATGCTCTGCATGAAACAGCCGTTCAGCTTATTGATCTCCCGGAAAGGCTGGCGGCGCGAGGCGTTCCCGAACA
GATAATGGAAATGCCCGCGATGGGTTTTCGCTACATCCCGGATAAACTTTCCCGTTGGGGTTTATTATGA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16185.64 Da        Isoelectric Point: 10.5154

>AT99756 WP_003859317.1 NZ_CP027604:4029709-4030146 [Enterobacter cloacae complex sp.]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEEPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Download         Length: 438 bp

>AT99756 NZ_CP027604:4029709-4030146 [Enterobacter cloacae complex sp.]
ATGAAAACACCTAATGAAGCTGAATCAGAGCTTTTACAGACATTAGCTCAAGTACGCAGCGTAAATAAAAAGCGACATCA
TGACGAGGCGGAAGAGCCGGATAAGCCATCGGTAGTGAAGAGGCAACGCGTTACACAAGGCTTAACGCGGATTAGTACTC
TCGATCGCCAGGCTGTACTGCATGCAGCCATACGGGACATTTTGCTGGGGAAAATCACACAGGGAGAGGCGCTGAAAAGG
CTCAGGGTCGAGGTGTTAGGGCTGAAGCAGGATGAATATGCAAAGCTGGTCAGCGTATCCCGGAAAACATTGTCGGATGT
AGAAAATAACAGAGGCAATTATTCCGCTGACGTCATAAATAAAATCTTTAAACCCTTTGGGCTTCAAACCGGACTAGTGC
CCGTTTCAAAATCGCTTATCGCTTCACTTTTCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5ZXW9

Antitoxin

Source ID Structure
AlphaFold DB A0A837FFV9

References