99747

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3206934..3207673 Replicon chromosome
Accession NZ_CP027604
Organism Enterobacter cloacae complex sp. strain AR_0093

Toxin (Protein)


Gene name tacT Uniprot ID A0A3L9PBP9
Locus tag AM472_RS15695 Protein ID WP_003857133.1
Coordinates 3206934..3207419 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag AM472_RS15700 Protein ID WP_003857131.1
Coordinates 3207407..3207673 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM472_RS15680 (AM472_15665) 3203801..3205234 - 1434 WP_004016055.1 restriction endonuclease -
AM472_RS15685 (AM472_15670) 3205255..3206058 - 804 WP_039268887.1 TIGR02391 family protein -
AM472_RS15690 (AM472_15675) 3206080..3206559 - 480 WP_001542736.1 hypothetical protein -
AM472_RS15695 (AM472_15680) 3206934..3207419 - 486 WP_003857133.1 GNAT family N-acetyltransferase Toxin
AM472_RS15700 (AM472_15685) 3207407..3207673 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
AM472_RS15705 (AM472_15690) 3207737..3208666 - 930 WP_022651200.1 LysR family transcriptional regulator -
AM472_RS15710 (AM472_15695) 3208796..3210184 + 1389 WP_039268889.1 MFS transporter -
AM472_RS15715 (AM472_15700) 3210206..3211201 - 996 WP_003857125.1 DUF2891 domain-containing protein -
AM472_RS15720 (AM472_15705) 3211211..3212197 - 987 WP_022651202.1 DUF979 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3183076..3207673 24597


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(58-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17540.23 Da        Isoelectric Point: 9.9658

>T99747 WP_003857133.1 NZ_CP027604:c3207419-3206934 [Enterobacter cloacae complex sp.]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T99747 NZ_CP027604:c3207419-3206934 [Enterobacter cloacae complex sp.]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACCTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGGAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCAGTTGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT99747 WP_003857131.1 NZ_CP027604:c3207673-3207407 [Enterobacter cloacae complex sp.]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT99747 NZ_CP027604:c3207673-3207407 [Enterobacter cloacae complex sp.]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCGGCCATAGAAAAACTGCTG
GCAAGGAAACCACAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3L9PBP9

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References