Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1995960..1996580 Replicon chromosome
Accession NZ_CP027604
Organism Enterobacter cloacae complex sp. strain AR_0093

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag AM472_RS09705 Protein ID WP_015571250.1
Coordinates 1995960..1996178 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag AM472_RS09710 Protein ID WP_006809850.1
Coordinates 1996206..1996580 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM472_RS09675 (AM472_09660) 1991972..1992232 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
AM472_RS09680 (AM472_09665) 1992235..1992375 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM472_RS09685 (AM472_09670) 1992372..1993082 - 711 WP_022650493.1 GNAT family protein -
AM472_RS09690 (AM472_09675) 1993184..1994644 + 1461 WP_022650494.1 PLP-dependent aminotransferase family protein -
AM472_RS09695 (AM472_09680) 1994616..1995083 - 468 WP_022650495.1 YlaC family protein -
AM472_RS09700 (AM472_09685) 1995200..1995751 - 552 WP_022650496.1 maltose O-acetyltransferase -
AM472_RS09705 (AM472_09690) 1995960..1996178 - 219 WP_015571250.1 HHA domain-containing protein Toxin
AM472_RS09710 (AM472_09695) 1996206..1996580 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
AM472_RS09715 (AM472_09700) 1997091..2000237 - 3147 WP_022650497.1 multidrug efflux RND transporter permease subunit AcrB -
AM472_RS09720 (AM472_09705) 2000260..2001453 - 1194 WP_022650498.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T99745 WP_015571250.1 NZ_CP027604:c1996178-1995960 [Enterobacter cloacae complex sp.]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T99745 NZ_CP027604:c1996178-1995960 [Enterobacter cloacae complex sp.]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT99745 WP_006809850.1 NZ_CP027604:c1996580-1996206 [Enterobacter cloacae complex sp.]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT99745 NZ_CP027604:c1996580-1996206 [Enterobacter cloacae complex sp.]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References