Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 1211997..1212593 Replicon chromosome
Accession NZ_CP027604
Organism Enterobacter cloacae complex sp. strain AR_0093

Toxin (Protein)


Gene name PumA Uniprot ID A0A5D9Q9B8
Locus tag AM472_RS05915 Protein ID WP_003860348.1
Coordinates 1212291..1212593 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A837FG03
Locus tag AM472_RS05910 Protein ID WP_022650216.1
Coordinates 1211997..1212284 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM472_RS05905 (AM472_05900) 1210369..1212000 + 1632 WP_003860350.1 Na/Pi cotransporter family protein -
AM472_RS05910 (AM472_05905) 1211997..1212284 - 288 WP_022650216.1 putative addiction module antidote protein Antitoxin
AM472_RS05915 (AM472_05910) 1212291..1212593 - 303 WP_003860348.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM472_RS05920 (AM472_05915) 1212791..1213663 + 873 WP_003860347.1 23S rRNA pseudouridine(2604) synthase RluF -
AM472_RS05925 (AM472_05920) 1213664..1213936 - 273 WP_003860346.1 DUF3811 domain-containing protein -
AM472_RS05930 (AM472_05925) 1213987..1214931 - 945 WP_022650218.1 ketopantoate/pantoate/pantothenate transporter PanS -
AM472_RS05935 (AM472_05930) 1215025..1216374 - 1350 WP_003860344.1 lysine-sensitive aspartokinase 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-93)

Antitoxin

Uncharacterized protein HI1420

(3-90)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11393.20 Da        Isoelectric Point: 10.1042

>T99743 WP_003860348.1 NZ_CP027604:c1212593-1212291 [Enterobacter cloacae complex sp.]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHLGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Download         Length: 303 bp

>T99743 NZ_CP027604:c1212593-1212291 [Enterobacter cloacae complex sp.]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTGGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10087.57 Da        Isoelectric Point: 5.7807

>AT99743 WP_022650216.1 NZ_CP027604:c1212284-1211997 [Enterobacter cloacae complex sp.]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Download         Length: 288 bp

>AT99743 NZ_CP027604:c1212284-1211997 [Enterobacter cloacae complex sp.]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5D9Q9B8

Antitoxin

Source ID Structure
AlphaFold DB A0A837FG03

References