Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 47400..48043 Replicon plasmid unnamed2
Accession NZ_CP027601
Organism Escherichia coli strain 97-3250

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag C7A06_RS33670 Protein ID WP_001044768.1
Coordinates 47400..47816 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag C7A06_RS33675 Protein ID WP_001261287.1
Coordinates 47813..48043 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A06_RS33645 42562..43236 - 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
C7A06_RS33650 43290..43676 - 387 WP_001066949.1 tyrosine-type recombinase/integrase -
C7A06_RS33655 43804..44664 + 861 WP_000704534.1 alpha/beta hydrolase -
C7A06_RS34665 45279..45428 + 150 WP_000955366.1 hypothetical protein -
C7A06_RS33665 46693..47238 + 546 WP_001165114.1 hypothetical protein -
C7A06_RS33670 47400..47816 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
C7A06_RS33675 47813..48043 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C7A06_RS33695 48603..49016 + 414 WP_000465041.1 hypothetical protein -
C7A06_RS33700 49018..49800 + 783 WP_001164205.1 site-specific integrase -
C7A06_RS33705 49972..50325 + 354 WP_000864810.1 colicin M immunity protein -
C7A06_RS33710 50333..50524 - 192 Protein_63 lipid II-degrading bacteriocin -
C7A06_RS33715 50528..50671 - 144 Protein_64 transcriptional regulator -
C7A06_RS33720 50738..52176 - 1439 Protein_65 enterohemolysin T1SS ABC transporter subunit EhxD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - espP / hlyD / hlyD / hlyB / hlyA / hlyC / toxB / toxB / toxB 1..92590 92590


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T99742 WP_001044768.1 NZ_CP027601:c47816-47400 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T99742 NZ_CP027601:c47816-47400 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT99742 WP_001261287.1 NZ_CP027601:c48043-47813 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT99742 NZ_CP027601:c48043-47813 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References