Detailed information of TA system TA099737

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5193275..5194107
• Replicon: chromosome
• Accession: NZ_CP027599
• Organism: Escherichia coli strain 97-3250

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: C7A06_RS28695
• Protein ID: WP_000854753.1
• Coordinates: 5193275..5193649 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: C7A06_RS28700
• Protein ID: WP_001315620.1
• Coordinates: 5193739..5194107 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A06_RS28650	5188490..5189596	+	1107	WP_001341302.1	N-acetylneuraminate epimerase	-
C7A06_RS28655	5189661..5190641	+	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
C7A06_RS28660	5190649..5191299	-	651	WP_001037966.1	HNH endonuclease	-
C7A06_RS34595	5192344..5192496	-	153	WP_001280445.1	hypothetical protein	-
C7A06_RS28685	5192581..5192778	-	198	WP_000839281.1	DUF957 domain-containing protein	-
C7A06_RS28690	5192790..5193278	-	489	WP_000777547.1	hypothetical protein	-
C7A06_RS28695	5193275..5193649	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
C7A06_RS28700	5193739..5194107	-	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C7A06_RS28705	5194270..5194491	-	222	WP_000692345.1	DUF987 domain-containing protein	-
C7A06_RS28710	5194554..5195030	-	477	WP_001186774.1	RadC family protein	-
C7A06_RS28715	5195046..5195531	-	486	WP_000849588.1	antirestriction protein	-
C7A06_RS28720	5195586..5196404	-	819	WP_001234622.1	DUF945 domain-containing protein	-
C7A06_RS28730	5196504..5196737	-	234	WP_001119729.1	DUF905 family protein	-
C7A06_RS28735	5196816..5197271	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T99737 WP_000854753.1 NZ_CP027599:c5193649-5193275 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T99737 NZ_CP027599:c5193649-5193275 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT99737 WP_001315620.1 NZ_CP027599:c5194107-5193739 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT99737 NZ_CP027599:c5194107-5193739 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5