Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3109569..3110207 Replicon chromosome
Accession NZ_CP027599
Organism Escherichia coli strain 97-3250

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C7A06_RS17000 Protein ID WP_000813794.1
Coordinates 3109569..3109745 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C7A06_RS17005 Protein ID WP_001270286.1
Coordinates 3109791..3110207 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A06_RS16980 3105188..3106402 - 1215 WP_001307190.1 BenE family transporter YdcO -
C7A06_RS16985 3106455..3106991 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C7A06_RS16990 3107064..3109025 + 1962 WP_001341357.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C7A06_RS16995 3109117..3109347 - 231 WP_000494244.1 YncJ family protein -
C7A06_RS17000 3109569..3109745 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C7A06_RS17005 3109791..3110207 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C7A06_RS17010 3110286..3111691 + 1406 Protein_3152 PLP-dependent aminotransferase family protein -
C7A06_RS17015 3111936..3113081 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
C7A06_RS17020 3113099..3114112 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C7A06_RS17025 3114113..3115054 + 942 WP_001251332.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3104000..3105148 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T99729 WP_000813794.1 NZ_CP027599:3109569-3109745 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T99729 NZ_CP027599:3109569-3109745 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT99729 WP_001270286.1 NZ_CP027599:3109791-3110207 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT99729 NZ_CP027599:3109791-3110207 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References