Detailed information of TA system TA099724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2847695..2848221
• Replicon: chromosome
• Accession: NZ_CP027599
• Organism: Escherichia coli strain 97-3250

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: C7A06_RS15490
• Protein ID: WP_000323025.1
• Coordinates: 2847934..2848221 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: C7A06_RS15485
• Protein ID: WP_000534858.1
• Coordinates: 2847695..2847934 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A06_RS15450	2842881..2843108	+	228	WP_000476993.1	transcriptional regulator	-
C7A06_RS15455	2843092..2843613	+	522	WP_000705349.1	hypothetical protein	-
C7A06_RS15460	2843594..2844559	+	966	WP_000054507.1	hypothetical protein	-
C7A06_RS15465	2844600..2845019	+	420	WP_001151195.1	DUF977 family protein	-
C7A06_RS15470	2845053..2846393	+	1341	WP_000589012.1	ISNCY family transposase	-
C7A06_RS15475	2846830..2847162	-	333	WP_001317460.1	FlxA-like family protein	-
C7A06_RS15480	2847365..2847670	-	306	WP_001326990.1	hypothetical protein	-
C7A06_RS15485	2847695..2847934	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
C7A06_RS15490	2847934..2848221	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
C7A06_RS15495	2848294..2848449	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
C7A06_RS15505	2848617..2848889	+	273	WP_012817871.1	hypothetical protein	-
C7A06_RS15510	2848891..2849946	+	1056	WP_001369253.1	DUF968 domain-containing protein	-
C7A06_RS15515	2849947..2850312	+	366	WP_000140024.1	RusA family crossover junction endodeoxyribonuclease	-
C7A06_RS15520	2850321..2850863	+	543	WP_000640017.1	DUF1133 family protein	-
C7A06_RS15525	2851095..2851292	+	198	WP_000917767.1	hypothetical protein	-
C7A06_RS15530	2851443..2852492	+	1050	WP_000611215.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / nleA/espI	2821943..2890802	68859

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T99724 WP_000323025.1 NZ_CP027599:2847934-2848221 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T99724 NZ_CP027599:2847934-2848221 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT99724 WP_000534858.1 NZ_CP027599:2847695-2847934 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT99724 NZ_CP027599:2847695-2847934 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8