Detailed information of TA system TA099723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2528419..2528890
• Replicon: chromosome
• Accession: NZ_CP027599
• Organism: Escherichia coli strain 97-3250

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: C7A06_RS13690
• Protein ID: WP_001303511.1
• Coordinates: 2528419..2528697 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: C7A06_RS13695
• Protein ID: WP_001302048.1
• Coordinates: 2528699..2528890 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A06_RS13665	2524069..2526720	-	2652	WP_000105101.1	exodeoxyribonuclease VIII	-
C7A06_RS13670	2526819..2527094	-	276	WP_001307773.1	hypothetical protein	-
C7A06_RS13675	2527168..2527344	-	177	WP_000245534.1	hypothetical protein	-
C7A06_RS13680	2527338..2527559	-	222	WP_000560218.1	killing protein KilR	-
C7A06_RS13685	2527980..2528132	-	153	WP_001427316.1	DUF1391 family protein	-
C7A06_RS13690	2528419..2528697	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C7A06_RS13695	2528699..2528890	-	192	WP_001302048.1	hypothetical protein	Antitoxin
C7A06_RS13700	2528911..2529282	-	372	WP_001169687.1	hypothetical protein	-
C7A06_RS13705	2529380..2529682	+	303	WP_000172738.1	transcriptional regulator	-
C7A06_RS13710	2529679..2530104	+	426	WP_000693943.1	Rha family transcriptional regulator	-
C7A06_RS13715	2530127..2531089	+	963	WP_001444941.1	helix-turn-helix domain-containing protein	-
C7A06_RS13720	2531096..2531837	+	742	Protein_2539	ATP-binding protein	-
C7A06_RS13725	2531863..2532624	+	762	WP_000450872.1	DUF1627 domain-containing protein	-
C7A06_RS13730	2532657..2532938	+	282	WP_000603384.1	DNA-binding protein	-
C7A06_RS13735	2532935..2533162	+	228	WP_000699809.1	hypothetical protein	-
C7A06_RS13740	2533155..2533466	+	312	WP_001289673.1	hypothetical protein	-
C7A06_RS13745	2533594..2533812	+	219	WP_000683609.1	DUF4014 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2515539..2585189	69650

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T99723 WP_001303511.1 NZ_CP027599:c2528697-2528419 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T99723 NZ_CP027599:c2528697-2528419 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT99723 WP_001302048.1 NZ_CP027599:c2528890-2528699 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT99723 NZ_CP027599:c2528890-2528699 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0