Detailed information of TA system TA099705

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 961833..962487
• Replicon: chromosome
• Accession: NZ_CP027599
• Organism: Escherichia coli strain 97-3250

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: C7A06_RS05205
• Protein ID: WP_000244777.1
• Coordinates: 962080..962487 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: C7A06_RS05200
• Protein ID: WP_000354043.1
• Coordinates: 961833..962099 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A06_RS05175	957002..957745	+	744	WP_044162519.1	SDR family oxidoreductase	-
C7A06_RS05180	957802..959235	-	1434	WP_001341859.1	6-phospho-beta-glucosidase BglA	-
C7A06_RS05185	959280..959591	+	312	WP_001182954.1	N(4)-acetylcytidine aminohydrolase	-
C7A06_RS05190	959755..960414	+	660	WP_000250274.1	hemolysin III family protein	-
C7A06_RS05195	960610..961590	-	981	WP_000886095.1	tRNA-modifying protein YgfZ	-
C7A06_RS05200	961833..962099	+	267	WP_000354043.1	FAD assembly factor SdhE	Antitoxin
C7A06_RS05205	962080..962487	+	408	WP_000244777.1	toxin CptA	Toxin
C7A06_RS05210	962527..963048	-	522	WP_001055874.1	flavodoxin FldB	-
C7A06_RS05215	963160..964056	+	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
C7A06_RS05220	964081..964791	+	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
C7A06_RS05225	964797..966530	+	1734	WP_000813220.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T99705 WP_000244777.1 NZ_CP027599:962080-962487 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T99705 NZ_CP027599:962080-962487 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10563.16 Da
• Isoelectric Point: 5.1599

>AT99705 WP_000354043.1 NZ_CP027599:961833-962099 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDLDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT99705 NZ_CP027599:961833-962099 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCTGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7