Detailed information of TA system TA099703

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 48819..49654
• Replicon: chromosome
• Accession: NZ_CP027599
• Organism: Escherichia coli strain 97-3250

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: C7A06_RS00285
• Protein ID: WP_000854821.1
• Coordinates: 48819..49196 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C7A06_RS00290
• Protein ID: WP_001285609.1
• Coordinates: 49286..49654 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A06_RS00250	44460..45383	-	924	WP_000535958.1	carboxylate/amino acid/amine transporter	-
C7A06_RS00255	45494..46678	-	1185	WP_001172895.1	sugar efflux transporter	-
C7A06_RS00260	47107..47235	-	129	Protein_46	virulence RhuM family protein	-
C7A06_RS00270	47473..48315	-	843	WP_032348719.1	DUF4942 domain-containing protein	-
C7A06_RS00275	48400..48597	-	198	WP_086795284.1	DUF957 domain-containing protein	-
C7A06_RS00280	48673..48822	-	150	Protein_49	hypothetical protein	-
C7A06_RS00285	48819..49196	-	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
C7A06_RS00290	49286..49654	-	369	WP_001285609.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C7A06_RS00295	49734..49955	-	222	Protein_52	DUF987 domain-containing protein	-
C7A06_RS00300	50042..50518	-	477	WP_001186768.1	RadC family protein	-
C7A06_RS00305	50533..51012	-	480	WP_000706979.1	antirestriction protein	-
C7A06_RS33905	51112..51252	+	141	WP_000997937.1	hypothetical protein	-
C7A06_RS00310	51290..52096	-	807	WP_001175154.1	DUF945 domain-containing protein	-
C7A06_RS00315	52186..52419	-	234	WP_001278287.1	DUF905 family protein	-
C7A06_RS00320	52425..53102	-	678	WP_001097301.1	hypothetical protein	-
C7A06_RS00325	53250..53930	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T99703 WP_000854821.1 NZ_CP027599:c49196-48819 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T99703 NZ_CP027599:c49196-48819 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13587.35 Da
• Isoelectric Point: 5.9562

>AT99703 WP_001285609.1 NZ_CP027599:c49654-49286 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPHHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT99703 NZ_CP027599:c49654-49286 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATTAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCACCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54