Detailed information of TA system TA099699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3727599..3728436
• Replicon: chromosome
• Accession: NZ_CP027591
• Organism: Escherichia coli strain 2014C-3011

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: C7A05_RS21515
• Protein ID: WP_062882216.1
• Coordinates: 3727894..3728436 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: C7A05_RS21510
• Protein ID: WP_001297137.1
• Coordinates: 3727599..3727910 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A05_RS21485	3722619..3723566	+	948	WP_001239454.1	cytochrome o ubiquinol oxidase subunit II	-
C7A05_RS21490	3723588..3725579	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
C7A05_RS21495	3725569..3726183	+	615	WP_000179835.1	cytochrome o ubiquinol oxidase subunit III	-
C7A05_RS21500	3726183..3726512	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C7A05_RS21505	3726524..3727414	+	891	WP_000971336.1	heme o synthase	-
C7A05_RS21510	3727599..3727910	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
C7A05_RS21515	3727894..3728436	+	543	WP_062882216.1	GNAT family N-acetyltransferase	Toxin
C7A05_RS21520	3728492..3729427	-	936	WP_062882230.1	sel1 repeat family protein	-
C7A05_RS21525	3729836..3731200	+	1365	WP_062882217.1	MFS transporter	-
C7A05_RS21530	3731328..3731819	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
C7A05_RS21535	3731987..3732898	+	912	WP_032204083.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19804.08 Da
• Isoelectric Point: 8.8918

>T99699 WP_062882216.1 NZ_CP027591:3727894-3728436 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSKIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T99699 NZ_CP027591:3727894-3728436 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAAAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT99699 WP_001297137.1 NZ_CP027591:3727599-3727910 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT99699 NZ_CP027591:3727599-3727910 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCAAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3