Detailed information of TA system TA099696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3064068..3064546
• Replicon: chromosome
• Accession: NZ_CP027591
• Organism: Escherichia coli strain 2014C-3011

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: C7A05_RS17980
• Protein ID: WP_001303876.1
• Coordinates: 3064259..3064546 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: C7A05_RS17975
• Protein ID: WP_000536233.1
• Coordinates: 3064068..3064259 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A05_RS17925	3059104..3059382	+	279	WP_074433979.1	hypothetical protein	-
C7A05_RS17930	3059384..3060433	+	1050	WP_001265133.1	DUF968 domain-containing protein	-
C7A05_RS17935	3060446..3060817	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
C7A05_RS17940	3060807..3061178	+	372	WP_000090265.1	antitermination protein	-
C7A05_RS17945	3061330..3062148	+	819	WP_000265267.1	CPBP family intramembrane metalloprotease	-
C7A05_RS17950	3062435..3062632	+	198	WP_000917737.1	hypothetical protein	-
C7A05_RS29795	3062767..3063192	+	426	WP_176465538.1	hypothetical protein	-
C7A05_RS17960	3063179..3063274	-	96	Protein_3135	Rha family transcriptional regulator	-
C7A05_RS17965	3063258..3063530	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
C7A05_RS17970	3063639..3064040	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
C7A05_RS17975	3064068..3064259	+	192	WP_000536233.1	hypothetical protein	Antitoxin
C7A05_RS17980	3064259..3064546	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C7A05_RS17985	3064823..3064978	+	156	WP_000379575.1	DUF1391 family protein	-
C7A05_RS17995	3065120..3065509	+	390	WP_032205089.1	hypothetical protein	-
C7A05_RS18000	3065696..3065881	-	186	WP_032205087.1	hypothetical protein	-
C7A05_RS18010	3066455..3066643	+	189	WP_000413705.1	cell division inhibition protein DicB	-
C7A05_RS18015	3066640..3066831	+	192	WP_001098307.1	DUF1482 family protein	-
C7A05_RS18020	3066925..3069360	+	2436	WP_106879261.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ompA	3055797..3092529	36732

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T99696 WP_001303876.1 NZ_CP027591:3064259-3064546 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T99696 NZ_CP027591:3064259-3064546 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT99696 WP_000536233.1 NZ_CP027591:3064068-3064259 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT99696 NZ_CP027591:3064068-3064259 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA