Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 703908..704562 Replicon chromosome
Accession NZ_CP027591
Organism Escherichia coli strain 2014C-3011

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C7A05_RS04850 Protein ID WP_000244777.1
Coordinates 704155..704562 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C7A05_RS04845 Protein ID WP_000354046.1
Coordinates 703908..704174 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A05_RS04820 699077..699820 + 744 WP_062882111.1 SDR family oxidoreductase -
C7A05_RS04825 699877..701310 - 1434 WP_062882117.1 6-phospho-beta-glucosidase BglA -
C7A05_RS04830 701355..701666 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C7A05_RS04835 701830..702489 + 660 WP_000250274.1 hemolysin III family protein -
C7A05_RS04840 702685..703665 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C7A05_RS04845 703908..704174 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C7A05_RS04850 704155..704562 + 408 WP_000244777.1 toxin CptA Toxin
C7A05_RS04855 704602..705123 - 522 WP_001055874.1 flavodoxin FldB -
C7A05_RS04860 705235..706131 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C7A05_RS04865 706155..706865 + 711 WP_024226239.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C7A05_RS04870 706871..708604 + 1734 WP_024226240.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T99682 WP_000244777.1 NZ_CP027591:704155-704562 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T99682 NZ_CP027591:704155-704562 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT99682 WP_000354046.1 NZ_CP027591:703908-704174 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT99682 NZ_CP027591:703908-704174 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References