Detailed information of TA system TA099679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 140295..140926
• Replicon: chromosome
• Accession: NZ_CP027591
• Organism: Escherichia coli strain 2014C-3011

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C7A05_RS01700
• Protein ID: WP_032203651.1
• Coordinates: 140295..140570 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: C7A05_RS01705
• Protein ID: WP_000593555.1
• Coordinates: 140567..140926 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A05_RS01685	135299..136366	+	1068	WP_000361467.1	HlyD family secretion protein	-
C7A05_RS01690	136363..139098	+	2736	WP_000149164.1	ribosome-associated ATPase/putative transporter RbbA	-
C7A05_RS01695	139098..140222	+	1125	WP_001216257.1	ABC transporter permease	-
C7A05_RS01700	140295..140570	+	276	WP_032203651.1	type II toxin-antitoxin system HicA family toxin	Toxin
C7A05_RS01705	140567..140926	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C7A05_RS01710	141046..141447	-	402	WP_062882695.1	nickel-responsive transcriptional regulator NikR	-
C7A05_RS01715	141453..142259	-	807	WP_062882694.1	nickel import ATP-binding protein NikE	-
C7A05_RS01720	142256..143020	-	765	WP_062882693.1	nickel import ATP-binding protein NikD	-
C7A05_RS01725	143020..143853	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
C7A05_RS01730	143850..144794	-	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	132537..141447	8910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10213.97 Da
• Isoelectric Point: 10.7076

>T99679 WP_032203651.1 NZ_CP027591:140295-140570 [Escherichia coli]
MRTKLQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T99679 NZ_CP027591:140295-140570 [Escherichia coli]
ATGAGAACCAAGTTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT99679 WP_000593555.1 NZ_CP027591:140567-140926 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT99679 NZ_CP027591:140567-140926 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6