Detailed information of TA system TA099678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 65075..65676
• Replicon: plasmid unnamed2
• Accession: NZ_CP027590
• Organism: Escherichia coli strain 2014C-3011

Toxin (Protein)

• Gene name: doc
• Uniprot ID: F0JYA5
• Locus tag: C7A05_RS00835
• Protein ID: WP_001216044.1
• Coordinates: 65296..65676 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: C7A05_RS00830
• Protein ID: WP_001190712.1
• Coordinates: 65075..65296 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A05_RS00775	60465..61052	+	588	WP_023442318.1	DUF551 domain-containing protein	-
C7A05_RS00780	61052..61333	+	282	WP_000002123.1	ASCH domain-containing protein	-
C7A05_RS00785	61357..61650	+	294	WP_000267997.1	hypothetical protein	-
C7A05_RS00790	61657..62031	+	375	WP_000988652.1	hypothetical protein	-
C7A05_RS00795	62013..62702	+	690	WP_000057456.1	hypothetical protein	-
C7A05_RS00800	62686..62970	+	285	WP_001142389.1	hypothetical protein	-
C7A05_RS00805	62970..63275	+	306	WP_000988220.1	hypothetical protein	-
C7A05_RS00810	63272..63796	+	525	WP_000159954.1	toprim domain-containing protein	-
C7A05_RS00815	63857..64072	-	216	WP_001048304.1	hypothetical protein	-
C7A05_RS00820	64103..64438	-	336	WP_001133672.1	hypothetical protein	-
C7A05_RS00825	64613..65002	+	390	WP_000506726.1	DNA repair protein	-
C7A05_RS00830	65075..65296	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C7A05_RS00835	65296..65676	+	381	WP_001216044.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C7A05_RS00840	65681..65860	+	180	WP_024224144.1	hypothetical protein	-
C7A05_RS00845	65888..66931	+	1044	WP_023442316.1	DUF968 domain-containing protein	-
C7A05_RS00850	67020..67472	+	453	WP_001326849.1	late promoter-activating protein	-
C7A05_RS00855	67558..68750	+	1193	Protein_84	terminase	-
C7A05_RS00860	68750..70234	+	1485	WP_000124159.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92449	92449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13544.28 Da
• Isoelectric Point: 5.6343

>T99678 WP_001216044.1 NZ_CP027590:65296-65676 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Nucleotide

• Length: 381 bp

>T99678 NZ_CP027590:65296-65676 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT99678 WP_001190712.1 NZ_CP027590:65075-65296 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT99678 NZ_CP027590:65075-65296 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A6Y6HMN5

Antitoxin

• AlphaFold DB: A0A829CJB6