Detailed information of TA system TA099672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 27035..27594
• Replicon: plasmid unnamed1
• Accession: NZ_CP027589
• Organism: Escherichia coli strain 2014C-3011

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: C7A05_RS00125
• Protein ID: WP_000421257.1
• Coordinates: 27035..27310 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C7A05_RS00130
• Protein ID: WP_062881718.1
• Coordinates: 27310..27594 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A05_RS00120	25605..26818	+	1214	WP_162829200.1	IS3 family transposase	-
C7A05_RS00125	27035..27310	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C7A05_RS00130	27310..27594	-	285	WP_062881718.1	ribbon-helix-helix domain-containing protein	Antitoxin
C7A05_RS00140	28506..29363	-	858	WP_096851680.1	incFII family plasmid replication initiator RepA	-
C7A05_RS00150	29830..29904	-	75	WP_001442103.1	RepA leader peptide Tap	-
C7A05_RS00160	30136..30393	-	258	WP_000083845.1	replication regulatory protein RepA	-
C7A05_RS00165	30677..30883	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	-
-	30870..30931	+	62	NuclAT_0	-	-
-	30870..30931	+	62	NuclAT_0	-	-
-	30870..30931	+	62	NuclAT_0	-	-
-	30870..30931	+	62	NuclAT_0	-	-
C7A05_RS29660	31186..31260	-	75	Protein_26	endonuclease	-
C7A05_RS00175	31506..31717	-	212	Protein_27	ANR family transcriptional regulator	-
C7A05_RS00180	31853..32412	-	560	Protein_28	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / espP	1..75065	75065

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T99672 WP_000421257.1 NZ_CP027589:c27310-27035 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T99672 NZ_CP027589:c27310-27035 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6471

>AT99672 WP_062881718.1 NZ_CP027589:c27594-27310 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLDALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT99672 NZ_CP027589:c27594-27310 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGATGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAAGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5