Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 54538..55181 Replicon plasmid unnamed
Accession NZ_CP027588
Organism Escherichia coli strain 2013C-4974

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag C6998_RS28355 Protein ID WP_001044768.1
Coordinates 54765..55181 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag C6998_RS28350 Protein ID WP_001261287.1
Coordinates 54538..54768 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6998_RS28320 50413..51852 + 1440 WP_001213546.1 enterohemolysin T1SS ABC transporter subunit EhxD -
C6998_RS28325 52044..52205 + 162 Protein_56 lipid II-degrading bacteriocin -
C6998_RS28330 52255..52608 - 354 WP_000864811.1 colicin M immunity protein -
C6998_RS28335 52780..53562 - 783 WP_001164190.1 site-specific integrase -
C6998_RS28340 53564..53977 - 414 WP_000465048.1 hypothetical protein -
C6998_RS28350 54538..54768 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C6998_RS28355 54765..55181 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
C6998_RS28360 55343..55648 - 306 WP_000376744.1 hypothetical protein -
C6998_RS28365 55757..56137 + 381 WP_001171554.1 IS66 family insertion sequence hypothetical protein -
C6998_RS28370 56134..56481 + 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
C6998_RS28375 56531..58069 + 1539 WP_000998050.1 IS66-like element ISEc8 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP / hlyC / hlyA / hlyB / hlyD 1..58109 58109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T99671 WP_001044768.1 NZ_CP027588:54765-55181 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T99671 NZ_CP027588:54765-55181 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT99671 WP_001261287.1 NZ_CP027588:54538-54768 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT99671 NZ_CP027588:54538-54768 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAATCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References