Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3792766..3793397 Replicon chromosome
Accession NZ_CP027587
Organism Escherichia coli strain 2013C-4974

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C6998_RS20680 Protein ID WP_001259385.1
Coordinates 3793122..3793397 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6998_RS20675 Protein ID WP_000593555.1
Coordinates 3792766..3793125 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6998_RS20650 3788898..3789842 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
C6998_RS20655 3789839..3790672 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
C6998_RS20660 3790672..3791436 + 765 WP_001136217.1 nickel import ATP-binding protein NikD -
C6998_RS20665 3791433..3792239 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
C6998_RS20670 3792245..3792646 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C6998_RS20675 3792766..3793125 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6998_RS20680 3793122..3793397 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C6998_RS20685 3793470..3794594 - 1125 WP_001216257.1 ABC transporter permease -
C6998_RS20690 3794594..3797329 - 2736 WP_097453223.1 ribosome-associated ATPase/putative transporter RbbA -
C6998_RS20695 3797326..3798393 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T99668 WP_001259385.1 NZ_CP027587:c3793397-3793122 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T99668 NZ_CP027587:c3793397-3793122 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT99668 WP_000593555.1 NZ_CP027587:c3793125-3792766 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT99668 NZ_CP027587:c3793125-3792766 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References