Detailed information of TA system TA099665

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3336909..3337746
• Replicon: chromosome
• Accession: NZ_CP027587
• Organism: Escherichia coli strain 2013C-4974

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: C6998_RS18115
• Protein ID: WP_000854687.1
• Coordinates: 3336909..3337289 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C6998_RS18120
• Protein ID: WP_012816826.1
• Coordinates: 3337378..3337746 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6998_RS18085	3332552..3334393	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
C6998_RS18090	3334471..3334977	-	507	WP_000228940.1	G/U mismatch-specific DNA glycosylase	-
C6998_RS18100	3335277..3336122	-	846	WP_001274558.1	DUF4942 domain-containing protein	-
C6998_RS18105	3336207..3336404	-	198	WP_000772026.1	DUF957 domain-containing protein	-
C6998_RS18110	3336424..3336912	-	489	WP_001054227.1	hypothetical protein	-
C6998_RS18115	3336909..3337289	-	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
C6998_RS18120	3337378..3337746	-	369	WP_012816826.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6998_RS18125	3337821..3338042	-	222	WP_000692340.1	DUF987 domain-containing protein	-
C6998_RS18130	3338111..3338587	-	477	WP_001364966.1	RadC family protein	-
C6998_RS18135	3338603..3339076	-	474	WP_001364892.1	antirestriction protein	-
C6998_RS18140	3339170..3339415	-	246	WP_000680588.1	hypothetical protein	-
C6998_RS18145	3339415..3340236	-	822	WP_001234566.1	DUF945 domain-containing protein	-
C6998_RS18155	3340456..3340866	-	411	WP_000848829.1	hypothetical protein	-
C6998_RS18160	3340882..3341559	-	678	WP_097453252.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T99665 WP_000854687.1 NZ_CP027587:c3337289-3336909 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T99665 NZ_CP027587:c3337289-3336909 [Escherichia coli]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13780.56 Da
• Isoelectric Point: 6.4763

>AT99665 WP_012816826.1 NZ_CP027587:c3337746-3337378 [Escherichia coli]
VSDTLHETNYPNDNHNRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT99665 NZ_CP027587:c3337746-3337378 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACAACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67