Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3216658..3217490 Replicon chromosome
Accession NZ_CP027587
Organism Escherichia coli strain 2013C-4974

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag C6998_RS17480 Protein ID WP_000854753.1
Coordinates 3217116..3217490 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID E3PJ72
Locus tag C6998_RS17475 Protein ID WP_001278232.1
Coordinates 3216658..3217026 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6998_RS17420 3211697..3211984 + 288 WP_000705928.1 PTS sugar transporter subunit IIB -
C6998_RS17425 3211997..3212206 + 210 Protein_3190 PTS ascorbate transporter subunit IIC -
C6998_RS17430 3212257..3213470 + 1214 WP_167579206.1 IS3 family transposase -
C6998_RS28850 3213515..3215076 + 1562 Protein_3192 hypothetical protein -
C6998_RS17450 3215152..3215607 + 456 WP_000581504.1 hypothetical protein -
C6998_RS17455 3215686..3215919 + 234 WP_001119721.1 DUF905 family protein -
C6998_RS28855 3216019..3216123 + 105 Protein_3195 DUF945 domain-containing protein -
C6998_RS17465 3216119..3216211 + 93 Protein_3196 hypothetical protein -
C6998_RS17470 3216274..3216495 + 222 WP_000692329.1 DUF987 domain-containing protein -
C6998_RS17475 3216658..3217026 + 369 WP_001278232.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
C6998_RS17480 3217116..3217490 + 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
C6998_RS17485 3217487..3217975 + 489 WP_000777541.1 hypothetical protein -
C6998_RS17490 3217987..3218184 + 198 WP_000839281.1 DUF957 domain-containing protein -
C6998_RS17495 3218281..3218850 + 570 WP_001290247.1 DUF4942 domain-containing protein -
C6998_RS17500 3219131..3219667 - 537 WP_000942793.1 GspM family type II secretion system protein YghD -
C6998_RS17505 3219669..3220847 - 1179 WP_000094993.1 type II secretion system protein GspL -
C6998_RS17510 3220844..3221821 - 978 WP_000633205.1 type II secretion system minor pseudopilin GspK -
C6998_RS17515 3221824..3222423 - 600 WP_001255034.1 type II secretion system minor pseudopilin GspJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T99664 WP_000854753.1 NZ_CP027587:3217116-3217490 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T99664 NZ_CP027587:3217116-3217490 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13491.37 Da        Isoelectric Point: 6.6290

>AT99664 WP_001278232.1 NZ_CP027587:3216658-3217026 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT99664 NZ_CP027587:3216658-3217026 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB E3PJ72

References