Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3091261..3091915 Replicon chromosome
Accession NZ_CP027587
Organism Escherichia coli strain 2013C-4974

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C6998_RS16775 Protein ID WP_000244777.1
Coordinates 3091261..3091668 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6998_RS16780 Protein ID WP_000354046.1
Coordinates 3091649..3091915 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6998_RS16755 3087219..3088952 - 1734 WP_024226240.1 single-stranded-DNA-specific exonuclease RecJ -
C6998_RS16760 3088958..3089668 - 711 WP_000715227.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6998_RS16765 3089692..3090588 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6998_RS16770 3090700..3091221 + 522 WP_001055874.1 flavodoxin FldB -
C6998_RS16775 3091261..3091668 - 408 WP_000244777.1 toxin CptA Toxin
C6998_RS16780 3091649..3091915 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6998_RS16785 3092158..3093138 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C6998_RS16790 3093334..3093993 - 660 WP_000250274.1 hemolysin III family protein -
C6998_RS16795 3094157..3094468 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C6998_RS16800 3094513..3095946 + 1434 WP_057711174.1 6-phospho-beta-glucosidase BglA -
C6998_RS16805 3096003..3096746 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T99663 WP_000244777.1 NZ_CP027587:c3091668-3091261 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T99663 NZ_CP027587:c3091668-3091261 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTACGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT99663 WP_000354046.1 NZ_CP027587:c3091915-3091649 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT99663 NZ_CP027587:c3091915-3091649 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References