Detailed information of TA system TA099660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2611596..2612221
• Replicon: chromosome
• Accession: NZ_CP027587
• Organism: Escherichia coli strain 2013C-4974

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6998_RS14240
• Protein ID: WP_000911330.1
• Coordinates: 2611596..2611994 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6998_RS14245
• Protein ID: WP_000450524.1
• Coordinates: 2611994..2612221 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6998_RS14220	2607474..2607674	+	201	WP_000383836.1	YpfN family protein	-
C6998_RS14225	2607784..2608482	-	699	WP_000679809.1	esterase	-
C6998_RS14230	2608556..2610571	-	2016	WP_000829335.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6998_RS14235	2610586..2611449	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
C6998_RS14240	2611596..2611994	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6998_RS14245	2611994..2612221	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6998_RS14250	2612375..2613088	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6998_RS14255	2613301..2614335	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
C6998_RS14260	2614352..2615230	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6998_RS14265	2615376..2615948	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6998_RS14270	2615948..2616418	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T99660 WP_000911330.1 NZ_CP027587:c2611994-2611596 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T99660 NZ_CP027587:c2611994-2611596 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT99660 WP_000450524.1 NZ_CP027587:c2612221-2611994 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT99660 NZ_CP027587:c2612221-2611994 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33