Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1680364..1681002 Replicon chromosome
Accession NZ_CP027587
Organism Escherichia coli strain 2013C-4974

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C6998_RS09155 Protein ID WP_000813794.1
Coordinates 1680826..1681002 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C6998_RS09150 Protein ID WP_077886810.1
Coordinates 1680364..1680780 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6998_RS09130 1675515..1676456 - 942 WP_001251313.1 ABC transporter permease -
C6998_RS09135 1676457..1677470 - 1014 WP_097453357.1 ABC transporter ATP-binding protein -
C6998_RS09140 1677488..1678633 - 1146 WP_000047457.1 ABC transporter substrate-binding protein -
C6998_RS09145 1678878..1680285 - 1408 Protein_1661 PLP-dependent aminotransferase family protein -
C6998_RS09150 1680364..1680780 - 417 WP_077886810.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C6998_RS09155 1680826..1681002 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C6998_RS09160 1681224..1681454 + 231 WP_000494244.1 YncJ family protein -
C6998_RS09165 1681546..1683507 - 1962 WP_024177737.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C6998_RS09170 1683580..1684116 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C6998_RS09175 1684169..1685383 + 1215 WP_071796541.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-67)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T99658 WP_000813794.1 NZ_CP027587:c1681002-1680826 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T99658 NZ_CP027587:c1681002-1680826 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15276.66 Da        Isoelectric Point: 4.7386

>AT99658 WP_077886810.1 NZ_CP027587:c1680780-1680364 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDTVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT99658 NZ_CP027587:c1680780-1680364 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACACCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References