Detailed information of TA system TA099647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 151946..152783
• Replicon: chromosome
• Accession: NZ_CP027587
• Organism: Escherichia coli strain 2013C-4974

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A8E0FRI1
• Locus tag: C6998_RS00775
• Protein ID: WP_000227792.1
• Coordinates: 151946..152488 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: C6998_RS00780
• Protein ID: WP_001297137.1
• Coordinates: 152472..152783 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6998_RS00755	147485..148396	-	912	WP_000705865.1	2-dehydropantoate 2-reductase	-
C6998_RS00760	148564..149055	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
C6998_RS00765	149183..150547	-	1365	WP_001000952.1	MFS transporter	-
C6998_RS00770	150955..151890	+	936	WP_024177727.1	sel1 repeat family protein	-
C6998_RS00775	151946..152488	-	543	WP_000227792.1	GNAT family N-acetyltransferase	Toxin
C6998_RS00780	152472..152783	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
C6998_RS00785	152968..153858	-	891	WP_000971336.1	heme o synthase	-
C6998_RS00790	153870..154199	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C6998_RS00795	154199..154813	-	615	WP_000179835.1	cytochrome o ubiquinol oxidase subunit III	-
C6998_RS00800	154803..156794	-	1992	WP_097453220.1	cytochrome o ubiquinol oxidase subunit I	-
C6998_RS00805	156816..157763	-	948	WP_001239454.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19908.14 Da
• Isoelectric Point: 8.6178

>T99647 WP_000227792.1 NZ_CP027587:c152488-151946 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPRQKQFDCGNTVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T99647 NZ_CP027587:c152488-151946 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTAGGCA
AAAACAGTTTGATTGCGGTAACACTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT99647 WP_001297137.1 NZ_CP027587:c152783-152472 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT99647 NZ_CP027587:c152783-152472 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCAAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3