Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4784097..4784735 Replicon chromosome
Accession NZ_CP027586
Organism Escherichia coli strain 2012EL-2448

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C7A04_RS25480 Protein ID WP_000813794.1
Coordinates 4784097..4784273 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C7A04_RS25485 Protein ID WP_001270286.1
Coordinates 4784319..4784735 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A04_RS25460 4779716..4780930 - 1215 WP_001307190.1 BenE family transporter YdcO -
C7A04_RS25465 4780983..4781519 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C7A04_RS25470 4781592..4783553 + 1962 WP_024178299.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C7A04_RS25475 4783645..4783875 - 231 WP_000494244.1 YncJ family protein -
C7A04_RS25480 4784097..4784273 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C7A04_RS25485 4784319..4784735 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C7A04_RS25490 4784814..4786220 + 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
C7A04_RS25495 4786465..4787610 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
C7A04_RS25500 4787628..4788641 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C7A04_RS25505 4788642..4789582 + 941 Protein_4619 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4778411..4779676 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T99637 WP_000813794.1 NZ_CP027586:4784097-4784273 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T99637 NZ_CP027586:4784097-4784273 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT99637 WP_001270286.1 NZ_CP027586:4784319-4784735 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT99637 NZ_CP027586:4784319-4784735 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References